TY - GEN
T1 - 32-Channel single photon counting module for ultra-sensitive detection of DNA sequences
AU - Gudkov, Georgiy
AU - Dhulla, Vinit
AU - Borodin, Anatoly
AU - Gavrilov, Dmitri
AU - Stepoukhovitch, Andrey
AU - Tsupryk, Andriy
AU - Gorbovitski, Boris
AU - Gorfinkel, Vera
PY - 2006
Y1 - 2006
N2 - We continue our work on the design and implementation of multi-channel single photon detection systems for highly sensitive detection of ultra-weak fluorescence signals, for high-performance, multi-lane DNA sequencing instruments. A fiberized, 32-channel single photon detection (SPD) module based on single photon avalanche diode (SPAD), model C30902S-DTC, from Perkin Elmer Optoelectronics (PKI) has been designed and implemented. Unavailability of high performance, large area SPAD arrays and our desire to design high performance photon counting systems drives us to use individual diodes. Slight modifications in our quenching circuit has doubled the linear range of our system from 1MHz to 2MHz, which is the upper limit for these devices and the maximum saturation count rate has increased to 14 MHz. The detector module comprises of a single board computer PC-104 that enables data visualization, recording, processing, and transfer. Very low dark count (300-1000 counts/s), robust, efficient, simple data collection and processing, ease of connectivity to any other application demanding similar requirements and similar performance results to the best commercially available single photon counting module (SPCM from PKI) are some of the features of this system.
AB - We continue our work on the design and implementation of multi-channel single photon detection systems for highly sensitive detection of ultra-weak fluorescence signals, for high-performance, multi-lane DNA sequencing instruments. A fiberized, 32-channel single photon detection (SPD) module based on single photon avalanche diode (SPAD), model C30902S-DTC, from Perkin Elmer Optoelectronics (PKI) has been designed and implemented. Unavailability of high performance, large area SPAD arrays and our desire to design high performance photon counting systems drives us to use individual diodes. Slight modifications in our quenching circuit has doubled the linear range of our system from 1MHz to 2MHz, which is the upper limit for these devices and the maximum saturation count rate has increased to 14 MHz. The detector module comprises of a single board computer PC-104 that enables data visualization, recording, processing, and transfer. Very low dark count (300-1000 counts/s), robust, efficient, simple data collection and processing, ease of connectivity to any other application demanding similar requirements and similar performance results to the best commercially available single photon counting module (SPCM from PKI) are some of the features of this system.
KW - Avalanche photodiode (APD)
KW - C30902S-DTC
KW - DNA sequencing
KW - Multi-channel photon counting
KW - Multi-channel single-photon detection
KW - Multi-lane DNA sequencing
KW - Photon counting
KW - Single-photon avalanche diode (SPAD)
KW - Single-photon counting
KW - Single-photon detection
UR - https://www.scopus.com/pages/publications/33846236329
U2 - 10.1117/12.685838
DO - 10.1117/12.685838
M3 - Conference contribution
AN - SCOPUS:33846236329
SN - 0819464708
SN - 9780819464705
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Advanced Photon Counting Techniques
T2 - Advanced Photon Counting Techniques
Y2 - 1 October 2006 through 3 October 2006
ER -