8-amino-2'-deoxyguanosine incorporation into oligomeric DNA

Robert A. Rieger; Charles R. Iden; Elena Gonikberg; Francis Johnson

doi:10.1080/07328319908045595

8-amino-2'-deoxyguanosine incorporation into oligomeric DNA

Robert A. Rieger
, Charles R. Iden
, Elena Gonikberg
, Francis Johnson

Pharmacological Sciences

Stony Brook University
Albany Molecular Research

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

During the incorporation of 8-amino-dG into oligomeric DNA, the deprotection conditions previously recommended (28% ammonia at room temperature) do not effect complete removal of the dimethylaminomethylene protecting groups. At elevated temperatures oxidative degradation of the oligomer and exchange of ammonia with dimethylamine in the protecting group at C8 occurred. The resolution of these problems and a method to obtain a series of homogenous oligomers in reasonable yield containing 8-amino-dG located site-specifically are described.

Original language	English
Pages (from-to)	73-88
Number of pages	16
Journal	Nucleosides and Nucleotides
Volume	18
Issue number	1
DOIs	https://doi.org/10.1080/07328319908045595
State	Published - 1999

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title = "8-amino-2'-deoxyguanosine incorporation into oligomeric DNA",

abstract = "During the incorporation of 8-amino-dG into oligomeric DNA, the deprotection conditions previously recommended (28\% ammonia at room temperature) do not effect complete removal of the dimethylaminomethylene protecting groups. At elevated temperatures oxidative degradation of the oligomer and exchange of ammonia with dimethylamine in the protecting group at C8 occurred. The resolution of these problems and a method to obtain a series of homogenous oligomers in reasonable yield containing 8-amino-dG located site-specifically are described.",

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year = "1999",

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T1 - 8-amino-2'-deoxyguanosine incorporation into oligomeric DNA

AU - Rieger, Robert A.

AU - Iden, Charles R.

AU - Gonikberg, Elena

AU - Johnson, Francis

PY - 1999

Y1 - 1999

N2 - During the incorporation of 8-amino-dG into oligomeric DNA, the deprotection conditions previously recommended (28% ammonia at room temperature) do not effect complete removal of the dimethylaminomethylene protecting groups. At elevated temperatures oxidative degradation of the oligomer and exchange of ammonia with dimethylamine in the protecting group at C8 occurred. The resolution of these problems and a method to obtain a series of homogenous oligomers in reasonable yield containing 8-amino-dG located site-specifically are described.

AB - During the incorporation of 8-amino-dG into oligomeric DNA, the deprotection conditions previously recommended (28% ammonia at room temperature) do not effect complete removal of the dimethylaminomethylene protecting groups. At elevated temperatures oxidative degradation of the oligomer and exchange of ammonia with dimethylamine in the protecting group at C8 occurred. The resolution of these problems and a method to obtain a series of homogenous oligomers in reasonable yield containing 8-amino-dG located site-specifically are described.

UR - https://www.scopus.com/pages/publications/0032957181

U2 - 10.1080/07328319908045595

DO - 10.1080/07328319908045595

M3 - Article

C2 - 10048224

AN - SCOPUS:0032957181

SN - 0732-8311

VL - 18

SP - 73

EP - 88

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 1

ER -

8-amino-2'-deoxyguanosine incorporation into oligomeric DNA

Abstract

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