A family of novel DNA sequencing instruments based on single-photon detection

Ludmila Alaverdian; Sinok Alaverdian; Olga Bilenko; Iouri Bogdanov; Elena Filippova; Dmitry Gavrilov; Boris Gorbovitski; Michael Gouzman; Georgy Gudkov; Sergey Domratchev; Olga Kosobokova; Nadia Lifshitz; Serge Luryi; Victor Ruskovoloshin; Andrew Stepoukhovitch; Marina Tcherevishnick; Georgy Tyshko; Vera Gorfinkel

doi:10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9

A family of novel DNA sequencing instruments based on single-photon detection

Ludmila Alaverdian
, Sinok Alaverdian
, Olga Bilenko
, Iouri Bogdanov
, Elena Filippova
, Dmitry Gavrilov
, Boris Gorbovitski
, Michael Gouzman
, Georgy Gudkov
, Sergey Domratchev
, Olga Kosobokova
, Nadia Lifshitz
, Serge Luryi
, Victor Ruskovoloshin
, Andrew Stepoukhovitch
, Marina Tcherevishnick
, Georgy Tyshko
, Vera Gorfinkel

Electrical Engineering

Stony Brook University
BioPhotonics Corporation

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.

Original language	English
Pages (from-to)	2804-2817
Number of pages	14
Journal	Electrophoresis
Volume	23
Issue number	16
DOIs	https://doi.org/10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9
State	Published - Aug 2002

Keywords

DNA sequencing instruments
Single-photon detection

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10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9

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Alaverdian, L., Alaverdian, S., Bilenko, O., Bogdanov, I., Filippova, E., Gavrilov, D., Gorbovitski, B., Gouzman, M., Gudkov, G., Domratchev, S., Kosobokova, O., Lifshitz, N., Luryi, S., Ruskovoloshin, V., Stepoukhovitch, A., Tcherevishnick, M., Tyshko, G., & Gorfinkel, V. (2002). A family of novel DNA sequencing instruments based on single-photon detection. Electrophoresis, 23(16), 2804-2817. https://doi.org/10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9

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abstract = "We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the {"}color-blind{"} single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.",

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author = "Ludmila Alaverdian and Sinok Alaverdian and Olga Bilenko and Iouri Bogdanov and Elena Filippova and Dmitry Gavrilov and Boris Gorbovitski and Michael Gouzman and Georgy Gudkov and Sergey Domratchev and Olga Kosobokova and Nadia Lifshitz and Serge Luryi and Victor Ruskovoloshin and Andrew Stepoukhovitch and Marina Tcherevishnick and Georgy Tyshko and Vera Gorfinkel",

year = "2002",

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Alaverdian, L, Alaverdian, S, Bilenko, O, Bogdanov, I, Filippova, E, Gavrilov, D, Gorbovitski, B, Gouzman, M, Gudkov, G, Domratchev, S, Kosobokova, O, Lifshitz, N, Luryi, S, Ruskovoloshin, V, Stepoukhovitch, A, Tcherevishnick, M, Tyshko, G & Gorfinkel, V 2002, 'A family of novel DNA sequencing instruments based on single-photon detection', Electrophoresis, vol. 23, no. 16, pp. 2804-2817. https://doi.org/10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9

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T1 - A family of novel DNA sequencing instruments based on single-photon detection

AU - Alaverdian, Ludmila

AU - Alaverdian, Sinok

AU - Bilenko, Olga

AU - Bogdanov, Iouri

AU - Filippova, Elena

AU - Gavrilov, Dmitry

AU - Gorbovitski, Boris

AU - Gouzman, Michael

AU - Gudkov, Georgy

AU - Domratchev, Sergey

AU - Kosobokova, Olga

AU - Lifshitz, Nadia

AU - Luryi, Serge

AU - Ruskovoloshin, Victor

AU - Stepoukhovitch, Andrew

AU - Tcherevishnick, Marina

AU - Tyshko, Georgy

AU - Gorfinkel, Vera

PY - 2002/8

Y1 - 2002/8

N2 - We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.

AB - We have developed a family of high-performance capillary DNA sequencing instruments based on a novel multicolor fluorescent detection technology. This technology is based on two technical innovations: the multilaser excitation of fluorescence of labeled DNA fragments and the "color-blind" single-photon detection of modulated fluorescence. Our machines employ modern digital and broadband techniques that are essential for achieving superior instrument performance. We discuss the design and testing results for several versions of the automated single lane DNA sequencers, as well as our approach to scaling up to multilane instruments.

KW - DNA sequencing instruments

KW - Single-photon detection

UR - https://www.scopus.com/pages/publications/0036672780

U2 - 10.1002/1522-2683(200208)23:16<2804::AID-ELPS2804>3.0.CO;2-9

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AN - SCOPUS:0036672780

SN - 0173-0835

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JO - Electrophoresis

JF - Electrophoresis

IS - 16

ER -

A family of novel DNA sequencing instruments based on single-photon detection

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