@inproceedings{7633e6ea7e1d4c69918c9203f1e3820b,
title = "A novel oxygen tension programmable microfluidic system (oPROMs) for in vitro cell biology studies",
abstract = "Creating different oxygen tensions at a microscale level can be beneficial for studies that investigate oxygen-dependent cellular behavior. Here we present a novel, highly flexible, oxygen tension programmable microfluidic system (oPROMs) capable of generating various uniform oxygen tensions without using complex device structures and multiple gas interconnections. The oPROMs device is coated with a mixture of Glucose oxidase (GOX) and Catalase (CAT) immobilized in a chitosan matrix and then covered by membranes with different thickness. The high glucose medium used in cell culture interacts with the enzyme layer and produces a steady hypoxia inside the wells. Based on different combination of GOX and CAT, oxygen tensions from 0\% to 7\% were produced.",
keywords = "cell culture device, hypoxia, microfluidic, oxygen gradient, Oxygen tension",
author = "N. Rajan and A. Narayan and Z. Wu and P. Wu and Ahn, \{C. H.\} and Narayan, \{R. K.\} and C. Li",
year = "2013",
doi = "10.1109/Transducers.2013.6626790",
language = "English",
isbn = "9781467359818",
series = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",
pages = "412--415",
booktitle = "2013 Transducers and Eurosensors XXVII",
note = "2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",
}