A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans

Chengda Zhang; James B. Konopka

doi:10.1128/EC.00327-09

A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans

Chengda Zhang
, James B. Konopka

Microbiology and Immunology

Stony Brook University

Research output: Contribution to journal › Comment/debate

56 Scopus citations

Abstract

Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.

Original language	English
Pages (from-to)	224-226
Number of pages	3
Journal	Eukaryotic Cell
Volume	9
Issue number	1
DOIs	https://doi.org/10.1128/EC.00327-09
State	Published - Jan 2010

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title = "A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans",

abstract = "Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.",

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AB - Fusions to the green fluorescent protein (GFP) are an effective way to monitor protein localization. However, altered codon usage in Candida species has delayed implementation of new variants. Examination of three new GFP variants in Candida albicans showed that one has higher signal intensity and increased resistance to photobleaching.

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M3 - Comment/debate

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JO - Eukaryotic Cell

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A photostable green fluorescent protein variant for analysis of protein localization in Candida albicans

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