A quick method for purifying bile salt-activated lipases

Andrew N.J. Moore; Helen Vakos; Tracey A.M. Neville; Peter Tonge; Prabhat Arya; Graham W. Burton

doi:10.1007/BF00159518

A quick method for purifying bile salt-activated lipases

Andrew N.J. Moore
, Helen Vakos
, Tracey A.M. Neville
, Peter Tonge
, Prabhat Arya
, Graham W. Burton

Chemistry

National Research Council of Canada
University of Ottawa

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A simple, one step chromatography procedure of general utility for purifying bile salt-activated upases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.

Original language	English
Pages (from-to)	523-528
Number of pages	6
Journal	Biotechnology Techniques
Volume	10
Issue number	7
DOIs	https://doi.org/10.1007/BF00159518
State	Published - 1996

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10.1007/BF00159518

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@article{4d06ce1d3add44eca9be557b33616f2e,

title = "A quick method for purifying bile salt-activated lipases",

abstract = "A simple, one step chromatography procedure of general utility for purifying bile salt-activated upases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.",

author = "Moore, \{Andrew N.J.\} and Helen Vakos and Neville, \{Tracey A.M.\} and Peter Tonge and Prabhat Arya and Burton, \{Graham W.\}",

year = "1996",

doi = "10.1007/BF00159518",

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pages = "523--528",

journal = "Biotechnology Techniques",

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TY - JOUR

T1 - A quick method for purifying bile salt-activated lipases

AU - Moore, Andrew N.J.

AU - Vakos, Helen

AU - Neville, Tracey A.M.

AU - Tonge, Peter

AU - Arya, Prabhat

AU - Burton, Graham W.

PY - 1996

Y1 - 1996

N2 - A simple, one step chromatography procedure of general utility for purifying bile salt-activated upases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.

AB - A simple, one step chromatography procedure of general utility for purifying bile salt-activated upases (BAL; cholesterol esterases) using a cholate affinity column is described. A key feature of the method is the use of a taurocholate concentration gradient in the eluent. The method has been used successfully to purify bovine and porcine BAL from commercial crude sources and BAL from human milk whey.

UR - https://www.scopus.com/pages/publications/0000243168

U2 - 10.1007/BF00159518

DO - 10.1007/BF00159518

M3 - Article

AN - SCOPUS:0000243168

SN - 0951-208X

VL - 10

SP - 523

EP - 528

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 7

ER -

A quick method for purifying bile salt-activated lipases

Abstract

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