A rhelogical separator for very large DNA molecules

Ken A. Dill; Bruno H. Zimm

doi:10.1093/nar/7.3.735

A rhelogical separator for very large DNA molecules

Ken A. Dill
, Bruno H. Zimm

Laufer Center for Physical and Quantitative Biology

University of California at San Diego

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

We present a rheological separation method for DNA molecules in which their deformability is used to advantage. This is the "radial migration method"; here we present experimental verification of the principle, theory having been reported elsewhere. The main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact T2 DNA (1.25 × 108 daltons) can be made to migrate about three centimeters in less than three hours.

Original language	English
Pages (from-to)	735-749
Number of pages	15
Journal	Nucleic Acids Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1093/nar/7.3.735
State	Published - Oct 10 1979

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title = "A rhelogical separator for very large DNA molecules",

abstract = "We present a rheological separation method for DNA molecules in which their deformability is used to advantage. This is the {"}radial migration method{"}; here we present experimental verification of the principle, theory having been reported elsewhere. The main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact T2 DNA (1.25 × 108 daltons) can be made to migrate about three centimeters in less than three hours.",

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AU - Zimm, Bruno H.

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N2 - We present a rheological separation method for DNA molecules in which their deformability is used to advantage. This is the "radial migration method"; here we present experimental verification of the principle, theory having been reported elsewhere. The main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact T2 DNA (1.25 × 108 daltons) can be made to migrate about three centimeters in less than three hours.

AB - We present a rheological separation method for DNA molecules in which their deformability is used to advantage. This is the "radial migration method"; here we present experimental verification of the principle, theory having been reported elsewhere. The main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact T2 DNA (1.25 × 108 daltons) can be made to migrate about three centimeters in less than three hours.

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U2 - 10.1093/nar/7.3.735

DO - 10.1093/nar/7.3.735

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VL - 7

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JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 3

ER -

A rhelogical separator for very large DNA molecules

Abstract

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