A role for Phospholipase D in Drosophila embryonic cellularization

Mary LaLonde; Hilde Janssens; Suyong Yun; Juan Crosby; Olga Redina; Virginie Olive; Yelena M. Altshuller; Seok Yong Choi; Guangwei Du; J. Peter Gergen; Michael A. Frohman

doi:10.1186/1471-213X-6-60

A role for Phospholipase D in Drosophila embryonic cellularization

Mary LaLonde
, Hilde Janssens
, Suyong Yun
, Juan Crosby
, Olga Redina
, Virginie Olive
, Yelena M. Altshuller
, Seok Yong Choi
, Guangwei Du
, J. Peter Gergen
, Michael A. Frohman

Stony Brook University
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Background: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically- translocated Golgi-derived vesicles. Results: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. Conclusion: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.

Original language	English
Article number	60
Journal	BMC Developmental Biology
Volume	6
DOIs	https://doi.org/10.1186/1471-213X-6-60
State	Published - Dec 7 2006

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title = "A role for Phospholipase D in Drosophila embryonic cellularization",

abstract = "Background: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically- translocated Golgi-derived vesicles. Results: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. Conclusion: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.",

author = "Mary LaLonde and Hilde Janssens and Suyong Yun and Juan Crosby and Olga Redina and Virginie Olive and Altshuller, \{Yelena M.\} and Choi, \{Seok Yong\} and Guangwei Du and Gergen, \{J. Peter\} and Frohman, \{Michael A.\}",

year = "2006",

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doi = "10.1186/1471-213X-6-60",

language = "English",

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T1 - A role for Phospholipase D in Drosophila embryonic cellularization

AU - LaLonde, Mary

AU - Janssens, Hilde

AU - Yun, Suyong

AU - Crosby, Juan

AU - Redina, Olga

AU - Olive, Virginie

AU - Altshuller, Yelena M.

AU - Choi, Seok Yong

AU - Du, Guangwei

AU - Gergen, J. Peter

AU - Frohman, Michael A.

PY - 2006/12/7

Y1 - 2006/12/7

N2 - Background: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically- translocated Golgi-derived vesicles. Results: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. Conclusion: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.

AB - Background: Cellularization of the Drosophila embryo is an unusually synchronous form of cytokinesis in which polarized membrane extension proceeds in part through incorporation of new membrane via fusion of apically- translocated Golgi-derived vesicles. Results: We describe here involvement of the signaling enzyme Phospholipase D (Pld) in regulation of this developmental step. Functional analysis using gene targeting revealed that cellularization is hindered by the loss of Pld, resulting frequently in early embryonic developmental arrest. Mechanistically, chronic Pld deficiency causes abnormal Golgi structure and secretory vesicle trafficking. Conclusion: Our results suggest that Pld functions to promote trafficking of Golgi-derived fusion-competent vesicles during cellularization.

UR - https://www.scopus.com/pages/publications/33845547658

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DO - 10.1186/1471-213X-6-60

M3 - Article

C2 - 17156430

AN - SCOPUS:33845547658

SN - 1471-213X

VL - 6

JO - BMC Developmental Biology

JF - BMC Developmental Biology

M1 - 60

ER -

A role for Phospholipase D in Drosophila embryonic cellularization

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