TY - GEN
T1 - A Topological Comparison of the Fluorescence Imitating Brightfield Imaging and H&E Imaging
AU - Xu, Meiliong
AU - Anderson, Nate
AU - Levenson, Richard M.
AU - Prasanna, Prateek
AU - Chen, Chao
N1 - Publisher Copyright:
© The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.
PY - 2025
Y1 - 2025
N2 - Fluorescence Imitating Brightfield Imaging (FIBI) represents an innovative approach in microscopy, providing real-time, non-destructive imaging of tissue without the need for the preparation of thin sections mounted on glass slides. The non-destructive nature of the technology permits tissue preservation for downstream analysis, which makes FIBI a promising alternative to traditional hematoxylin and eosin (H&E) staining in histopathology. Previous research has shown that FIBI can identify morphological features with similar or, in some cases, higher quality compared with H&E images. To comprehensively quantify the advantages and limitations of FIBI in tissue visualization, we propose a novel framework for characterizing the topological difference of FIBI and H&E slide pairs. Experiments are performed on slide pairs of FIBI and H&E imaging of the same tissue area. The proposed approach shows that FIBI can make morphological structures, like vessels, more salient and holds great promise as a complementary technique to H&E, offering novel insights into tissue architecture and potentially improving histopathological diagnostic accuracy.
AB - Fluorescence Imitating Brightfield Imaging (FIBI) represents an innovative approach in microscopy, providing real-time, non-destructive imaging of tissue without the need for the preparation of thin sections mounted on glass slides. The non-destructive nature of the technology permits tissue preservation for downstream analysis, which makes FIBI a promising alternative to traditional hematoxylin and eosin (H&E) staining in histopathology. Previous research has shown that FIBI can identify morphological features with similar or, in some cases, higher quality compared with H&E images. To comprehensively quantify the advantages and limitations of FIBI in tissue visualization, we propose a novel framework for characterizing the topological difference of FIBI and H&E slide pairs. Experiments are performed on slide pairs of FIBI and H&E imaging of the same tissue area. The proposed approach shows that FIBI can make morphological structures, like vessels, more salient and holds great promise as a complementary technique to H&E, offering novel insights into tissue architecture and potentially improving histopathological diagnostic accuracy.
KW - FIBI Imaging
KW - H&E Imaging
KW - Topology
UR - https://www.scopus.com/pages/publications/85207643625
U2 - 10.1007/978-3-031-73967-5_12
DO - 10.1007/978-3-031-73967-5_12
M3 - Conference contribution
AN - SCOPUS:85207643625
SN - 9783031739668
T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
SP - 122
EP - 133
BT - Topology- and Graph-Informed Imaging Informatics - 1st International Workshop, TGI3 2024, Held in Conjunction with MICCAI 2024, Proceedings
A2 - Chen, Chao
A2 - Singh, Yash
A2 - Hu, Xiaoling
PB - Springer Science and Business Media Deutschland GmbH
T2 - 1st Workshop on Topology- and Graph- Informed Imaging Informatics, TGI3 2024, held in conjunction with the 27th International Conference on Medical Image Computing and Computer Assisted Intervention, MICCAI 2024
Y2 - 10 October 2024 through 10 October 2024
ER -