ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines

Petr Daniel; Kamila Balušíková; Radka Václavíková; Karolína Šeborová; Šárka Ransdorfová; Marie Valeriánová; Longfei Wei; Michael Jelínek; Tereza Tlapáková; Thomas Fleischer; Vessela N. Kristensen; Pavel Souček; Iwao Ojima; Jan Kovář

doi:10.3390/genes14020296

ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines

Petr Daniel
, Kamila Balušíková
, Radka Václavíková
, Karolína Šeborová
, Šárka Ransdorfová
, Marie Valeriánová
, Longfei Wei
, Michael Jelínek
, Tereza Tlapáková
, Thomas Fleischer
, Vessela N. Kristensen
, Pavel Souček
, Iwao Ojima
, Jan Kovář

Charles University
Czech National Institute of Public Health
Institute of Hematology and Blood Transfusion
Stony Brook University
University of Oslo

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.

Original language	English
Article number	296
Journal	Genes
Volume	14
Issue number	2
DOIs	https://doi.org/10.3390/genes14020296
State	Published - Feb 2023

Keywords

ABCB1
CpG methylation
MCF-7
TRIP6
breast cancer
cAMP response element
gene amplification

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10.3390/genes14020296

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Daniel, P., Balušíková, K., Václavíková, R., Šeborová, K., Ransdorfová, Š., Valeriánová, M., Wei, L., Jelínek, M., Tlapáková, T., Fleischer, T., Kristensen, V. N., Souček, P., Ojima, I., & Kovář, J. (2023). ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines. Genes, 14(2), Article 296. https://doi.org/10.3390/genes14020296

@article{c3e7cdf6053a4d1087b9830a41f1796e,

title = "ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines",

abstract = "A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.",

keywords = "ABCB1, CpG methylation, MCF-7, TRIP6, breast cancer, cAMP response element, gene amplification",

author = "Petr Daniel and Kamila Balu{\v s}{\'i}kov{\'a} and Radka V{\'a}clav{\'i}kov{\'a} and Karol{\'i}na {\v S}eborov{\'a} and {\v S}{\'a}rka Ransdorfov{\'a} and Marie Valeri{\'a}nov{\'a} and Longfei Wei and Michael Jel{\'i}nek and Tereza Tlap{\'a}kov{\'a} and Thomas Fleischer and Kristensen, \{Vessela N.\} and Pavel Sou{\v c}ek and Iwao Ojima and Jan Kov{\'a}{\v r}",

note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = feb,

doi = "10.3390/genes14020296",

language = "English",

volume = "14",

journal = "Genes",

issn = "2073-4425",

number = "2",

}

Daniel, P, Balušíková, K, Václavíková, R, Šeborová, K, Ransdorfová, Š, Valeriánová, M, Wei, L, Jelínek, M, Tlapáková, T, Fleischer, T, Kristensen, VN, Souček, P, Ojima, I & Kovář, J 2023, 'ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines', Genes, vol. 14, no. 2, 296. https://doi.org/10.3390/genes14020296

TY - JOUR

T1 - ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines

AU - Daniel, Petr

AU - Balušíková, Kamila

AU - Václavíková, Radka

AU - Šeborová, Karolína

AU - Ransdorfová, Šárka

AU - Valeriánová, Marie

AU - Wei, Longfei

AU - Jelínek, Michael

AU - Tlapáková, Tereza

AU - Fleischer, Thomas

AU - Kristensen, Vessela N.

AU - Souček, Pavel

AU - Ojima, Iwao

AU - Kovář, Jan

PY - 2023/2

Y1 - 2023/2

N2 - A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.

AB - A limited number of studies are devoted to regulating TRIP6 expression in cancer. Hence, we aimed to unveil the regulation of TRIP6 expression in MCF-7 breast cancer cells (with high TRIP6 expression) and taxane-resistant MCF-7 sublines (manifesting even higher TRIP6 expression). We found that TRIP6 transcription is regulated primarily by the cyclic AMP response element (CRE) in hypomethylated proximal promoters in both taxane-sensitive and taxane-resistant MCF-7 cells. Furthermore, in taxane-resistant MCF-7 sublines, TRIP6 co-amplification with the neighboring ABCB1 gene, as witnessed by fluorescence in situ hybridization (FISH), led to TRIP6 overexpression. Ultimately, we found high TRIP6 mRNA levels in progesterone receptor-positive breast cancer and samples resected from premenopausal women.

KW - ABCB1

KW - CpG methylation

KW - MCF-7

KW - TRIP6

KW - breast cancer

KW - cAMP response element

KW - gene amplification

UR - https://www.scopus.com/pages/publications/85148869540

U2 - 10.3390/genes14020296

DO - 10.3390/genes14020296

M3 - Article

AN - SCOPUS:85148869540

SN - 2073-4425

VL - 14

JO - Genes

JF - Genes

IS - 2

M1 - 296

ER -

ABCB1 Amplicon Contains Cyclic AMP Response Element-Driven TRIP6 Gene in Taxane-Resistant MCF-7 Breast Cancer Sublines

Abstract

Keywords

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