Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA)

Devin Camenares; Daniel P. Dulebohn; Anton Svetlanov; A. Wali Karzai

doi:10.1074/jbc.M113.503896

Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA)

Devin Camenares
, Daniel P. Dulebohn
, Anton Svetlanov
, A. Wali Karzai

Biochemistry and Cell Biology

Stony Brook University
National Institutes of Health

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Background: tmRNA and small protein B (SmpB) rescue stalled ribosomes through a template switching mechanism. Results: Changes to the SmpB hinge, SmpB C terminus, or tmRNA ORF affect ribosome rescue activity and accuracy. Conclusion: Proper positioning of SmpB and tmRNA make distinct and supplementary contributions to ribosome rescue. Significance: Template switching requires concerted action of distinct SmpB, tmRNA, and ribosomal determinants.

Original language	English
Pages (from-to)	30527-30542
Number of pages	16
Journal	Journal of Biological Chemistry
Volume	288
Issue number	42
DOIs	https://doi.org/10.1074/jbc.M113.503896
State	Published - Oct 18 2013

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10.1074/jbc.M113.503896

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title = "Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA)",

abstract = "Background: tmRNA and small protein B (SmpB) rescue stalled ribosomes through a template switching mechanism. Results: Changes to the SmpB hinge, SmpB C terminus, or tmRNA ORF affect ribosome rescue activity and accuracy. Conclusion: Proper positioning of SmpB and tmRNA make distinct and supplementary contributions to ribosome rescue. Significance: Template switching requires concerted action of distinct SmpB, tmRNA, and ribosomal determinants.",

author = "Devin Camenares and Dulebohn, \{Daniel P.\} and Anton Svetlanov and Karzai, \{A. Wali\}",

year = "2013",

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day = "18",

doi = "10.1074/jbc.M113.503896",

language = "English",

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journal = "Journal of Biological Chemistry",

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Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA). / Camenares, Devin; Dulebohn, Daniel P.; Svetlanov, Anton et al.
In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30527-30542.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA)

AU - Camenares, Devin

AU - Dulebohn, Daniel P.

AU - Svetlanov, Anton

AU - Karzai, A. Wali

PY - 2013/10/18

Y1 - 2013/10/18

N2 - Background: tmRNA and small protein B (SmpB) rescue stalled ribosomes through a template switching mechanism. Results: Changes to the SmpB hinge, SmpB C terminus, or tmRNA ORF affect ribosome rescue activity and accuracy. Conclusion: Proper positioning of SmpB and tmRNA make distinct and supplementary contributions to ribosome rescue. Significance: Template switching requires concerted action of distinct SmpB, tmRNA, and ribosomal determinants.

AB - Background: tmRNA and small protein B (SmpB) rescue stalled ribosomes through a template switching mechanism. Results: Changes to the SmpB hinge, SmpB C terminus, or tmRNA ORF affect ribosome rescue activity and accuracy. Conclusion: Proper positioning of SmpB and tmRNA make distinct and supplementary contributions to ribosome rescue. Significance: Template switching requires concerted action of distinct SmpB, tmRNA, and ribosomal determinants.

UR - https://www.scopus.com/pages/publications/84886928712

U2 - 10.1074/jbc.M113.503896

DO - 10.1074/jbc.M113.503896

M3 - Article

C2 - 23986442

AN - SCOPUS:84886928712

SN - 0021-9258

VL - 288

SP - 30527

EP - 30542

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 42

ER -

Active and accurate trans-translation requires distinct determinants in the C-terminal tail of SmpB protein and the mRNA-like domain of transfer messenger RNA (tmRNA)

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