An electrochemical probe of DNA stacking in an antisense oligonucleotide containing a C3′-endo-locked sugar

Elizabeth M. Boon; Jacqueline K. Barton; Pushpangadan I. Pradeepkumar; Johan Isaksson; Catherine Petit; Jyoti Chattopadhyaya

doi:10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6

An electrochemical probe of DNA stacking in an antisense oligonucleotide containing a C3′-endo-locked sugar

Elizabeth M. Boon
, Jacqueline K. Barton
, Pushpangadan I. Pradeepkumar
, Johan Isaksson
, Catherine Petit
, Jyoti Chattopadhyaya

Chemistry

California Institute of Technology
Uppsala University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The preferred base-stacking orientation of a conformationally constrained nucleotide (3′-endo-locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA-modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA-mediated charge transport through DNA-modified surfaces in characterizing small perturbations in DNA stacking and structure.

Original language	English
Pages (from-to)	3402-3405
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	41
Issue number	18
DOIs	https://doi.org/10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6
State	Published - Sep 16 2002

Keywords

Antisense oligonucleotides
Charge transfer
DNA
DNA-modified surfaces
Nucleotides

Access to Document

10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6

Cite this

@article{89f062bb4fcf4773a516a905bfede8d4,

title = "An electrochemical probe of DNA stacking in an antisense oligonucleotide containing a C3′-endo-locked sugar",

abstract = "The preferred base-stacking orientation of a conformationally constrained nucleotide (3′-endo-locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA-modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA-mediated charge transport through DNA-modified surfaces in characterizing small perturbations in DNA stacking and structure.",

keywords = "Antisense oligonucleotides, Charge transfer, DNA, DNA-modified surfaces, Nucleotides",

author = "Boon, \{Elizabeth M.\} and Barton, \{Jacqueline K.\} and Pradeepkumar, \{Pushpangadan I.\} and Johan Isaksson and Catherine Petit and Jyoti Chattopadhyaya",

year = "2002",

month = sep,

day = "16",

doi = "10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6",

language = "English",

volume = "41",

pages = "3402--3405",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

number = "18",

}

TY - JOUR

T1 - An electrochemical probe of DNA stacking in an antisense oligonucleotide containing a C3′-endo-locked sugar

AU - Boon, Elizabeth M.

AU - Barton, Jacqueline K.

AU - Pradeepkumar, Pushpangadan I.

AU - Isaksson, Johan

AU - Petit, Catherine

AU - Chattopadhyaya, Jyoti

PY - 2002/9/16

Y1 - 2002/9/16

N2 - The preferred base-stacking orientation of a conformationally constrained nucleotide (3′-endo-locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA-modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA-mediated charge transport through DNA-modified surfaces in characterizing small perturbations in DNA stacking and structure.

AB - The preferred base-stacking orientation of a conformationally constrained nucleotide (3′-endo-locked) within DNA/DNA and DNA/RNA duplexes (see picture) was probed by charge transport through DNA-modified gold electrode surfaces. The conformation of the sugar is seen to sensitively determine the local stacking of the duplex. These results illustrate the utility of DNA-mediated charge transport through DNA-modified surfaces in characterizing small perturbations in DNA stacking and structure.

KW - Antisense oligonucleotides

KW - Charge transfer

KW - DNA

KW - DNA-modified surfaces

KW - Nucleotides

UR - https://www.scopus.com/pages/publications/0037119722

U2 - 10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6

DO - 10.1002/1521-3773(20020916)41:18<3402::AID-ANIE3402>3.0.CO;2-6

M3 - Article

C2 - 12298045

AN - SCOPUS:0037119722

SN - 1433-7851

VL - 41

SP - 3402

EP - 3405

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 18

ER -

An electrochemical probe of DNA stacking in an antisense oligonucleotide containing a C3′-endo-locked sugar

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this