An improved method for the preparation of the phosphoramidites of modified 2'-deoxynucleotides: Incorporation of 8-oxo-2'-deoxy-7h-guanosine into synthetic oligomers

Veeraiah Bodepudi; Charles R. Iden; Francis Johnson

doi:10.1080/07328319108046660

An improved method for the preparation of the phosphoramidites of modified 2'-deoxynucleotides: Incorporation of 8-oxo-2'-deoxy-7h-guanosine into synthetic oligomers

Veeraiah Bodepudi
, Charles R. Iden
, Francis Johnson

Pharmacological Sciences

Stony Brook University

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

3 '-O-(Diisopropylamino-2-cyanoethoxyphosphiny1)-5'-O-(4,4'-dimethoxytrity1)-N²-isobutyry1-8-oxo-2'-deoxy-7H-guanosine (7) was synthesized and used for the introduction of an 8-oxo-2'-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.

Original language	English
Pages (from-to)	755-761
Number of pages	7
Journal	Nucleosides and Nucleotides
Volume	10
Issue number	4
DOIs	https://doi.org/10.1080/07328319108046660
State	Published - Jun 1 1991

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title = "An improved method for the preparation of the phosphoramidites of modified 2'-deoxynucleotides: Incorporation of 8-oxo-2'-deoxy-7h-guanosine into synthetic oligomers",

abstract = "3 '-O-(Diisopropylamino-2-cyanoethoxyphosphiny1)-5'-O-(4,4'-dimethoxytrity1)-N2-isobutyry1-8-oxo-2'-deoxy-7H-guanosine (7) was synthesized and used for the introduction of an 8-oxo-2'-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.",

author = "Veeraiah Bodepudi and Iden, \{Charles R.\} and Francis Johnson",

year = "1991",

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T1 - An improved method for the preparation of the phosphoramidites of modified 2'-deoxynucleotides

T2 - Incorporation of 8-oxo-2'-deoxy-7h-guanosine into synthetic oligomers

AU - Bodepudi, Veeraiah

AU - Iden, Charles R.

AU - Johnson, Francis

PY - 1991/6/1

Y1 - 1991/6/1

N2 - 3 '-O-(Diisopropylamino-2-cyanoethoxyphosphiny1)-5'-O-(4,4'-dimethoxytrity1)-N2-isobutyry1-8-oxo-2'-deoxy-7H-guanosine (7) was synthesized and used for the introduction of an 8-oxo-2'-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.

AB - 3 '-O-(Diisopropylamino-2-cyanoethoxyphosphiny1)-5'-O-(4,4'-dimethoxytrity1)-N2-isobutyry1-8-oxo-2'-deoxy-7H-guanosine (7) was synthesized and used for the introduction of an 8-oxo-2'-deoxy-7H-guanosine residue into a DNA oligomer by means of automated synthesis. A modification of the preparation of the phosphoramidite markedly improves the coupling efficiency in the oligomer synthesis in this and several other cases that were tested.

UR - https://www.scopus.com/pages/publications/0025797517

U2 - 10.1080/07328319108046660

DO - 10.1080/07328319108046660

M3 - Article

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SN - 0732-8311

VL - 10

SP - 755

EP - 761

JO - Nucleosides and Nucleotides

JF - Nucleosides and Nucleotides

IS - 4

ER -

An improved method for the preparation of the phosphoramidites of modified 2'-deoxynucleotides: Incorporation of 8-oxo-2'-deoxy-7h-guanosine into synthetic oligomers

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