@inbook{9bced686590c429b8c3c1b2843880021,
title = "Antibody Arrays: Barcode Technology",
abstract = "Antibody microarray is a fundamental, high-content technology for analyzing biomarkers with a multiplexity even at the proteomic level. Recent advancement in this field has driven the antibody array into a new territory related with single-cell analysis. Here we describe a flow pattern-based method for producing a high-density barcode antibody microarray for the detection of proteins in fluidic samples and in single cells. The antibody microarray is fabricated by a perpendicularly oriented flow patterning of single-stranded barcode DNAs, which are then converted into DNA-antibody conjugates. Compared to conventional microarrays, this barcode antibody microarray features a simple and high-throughput assay while achieving both high sensitivity and specificity. This barcode technology provides new clues for developing next-generation antibody microarrays and can be widely used in protein biomarker discovery, cell signaling network analysis, and disease diagnosis and prognosis.",
keywords = "Antibody array, Antibody-DNA conjugation, DNA barcoding, Microarray, Multiplex, Protein detection, Proteomics, Single-cell analysis",
author = "Liwei Yang and Jun Wang",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1064-0\_8",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "93--102",
booktitle = "Methods in Molecular Biology",
}