Application of KIE and thia approaches in the mechanistic study of a plant stearoyl-ACP Δ9 desaturase

B. Behrouzian; P. H. Buist; J. Shanklin

doi:10.1039/b008972i

Application of KIE and thia approaches in the mechanistic study of a plant stearoyl-ACP Δ⁹ desaturase

B. Behrouzian
, P. H. Buist
, J. Shanklin

Biochemistry and Cell Biology

Carleton University

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The mechanism of the castor stearoyl-ACP Δ⁹ desaturase has been investigated; it has been shown that C-H cleavage at carbons 9 and 10 is not sensitive to deuterium substitution and oxo-trapping experiments using thia analogues revealed that sulfoxidation occurs when the substrate bears sulfur at the 9 and 10 positions.

Original language	English
Pages (from-to)	401-402
Number of pages	2
Journal	Chemical Communications
Issue number	5
DOIs	https://doi.org/10.1039/b008972i
State	Published - Mar 7 2001

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title = "Application of KIE and thia approaches in the mechanistic study of a plant stearoyl-ACP Δ9 desaturase",

abstract = "The mechanism of the castor stearoyl-ACP Δ9 desaturase has been investigated; it has been shown that C-H cleavage at carbons 9 and 10 is not sensitive to deuterium substitution and oxo-trapping experiments using thia analogues revealed that sulfoxidation occurs when the substrate bears sulfur at the 9 and 10 positions.",

author = "B. Behrouzian and Buist, \{P. H.\} and J. Shanklin",

year = "2001",

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doi = "10.1039/b008972i",

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journal = "Chemical Communications",

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T1 - Application of KIE and thia approaches in the mechanistic study of a plant stearoyl-ACP Δ9 desaturase

AU - Behrouzian, B.

AU - Buist, P. H.

AU - Shanklin, J.

PY - 2001/3/7

Y1 - 2001/3/7

N2 - The mechanism of the castor stearoyl-ACP Δ9 desaturase has been investigated; it has been shown that C-H cleavage at carbons 9 and 10 is not sensitive to deuterium substitution and oxo-trapping experiments using thia analogues revealed that sulfoxidation occurs when the substrate bears sulfur at the 9 and 10 positions.

AB - The mechanism of the castor stearoyl-ACP Δ9 desaturase has been investigated; it has been shown that C-H cleavage at carbons 9 and 10 is not sensitive to deuterium substitution and oxo-trapping experiments using thia analogues revealed that sulfoxidation occurs when the substrate bears sulfur at the 9 and 10 positions.

UR - https://www.scopus.com/pages/publications/0035820053

U2 - 10.1039/b008972i

DO - 10.1039/b008972i

M3 - Article

AN - SCOPUS:0035820053

SN - 1359-7345

SP - 401

EP - 402

JO - Chemical Communications

JF - Chemical Communications

IS - 5

ER -

Application of KIE and thia approaches in the mechanistic study of a plant stearoyl-ACP Δ⁹ desaturase

Abstract

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