Application of o-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin

Qing Zhi Zhu; Xue Ying Zheng; Jin Gou Xu; Feng Hua Liu; Qing Ge Li

doi:10.1006/mchj.1997.1485

Application of o-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin

Qing Zhi Zhu
, Xue Ying Zheng
, Jin Gou Xu
, Feng Hua Liu
, Qing Ge Li

Office of the Dean SOMAS

Xiamen University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A highly sensitive fluorescence quenching method has been developed for selective determination of hydrogen peroxide based on the catalytic effect of hemin on the o-hydroxyphenylfluorone (a new fluorogenic substrate) and hydrogen peroxide system. Under optimum conditions, the calibration graph was linear over the range 0-1.0 × 10^-6 mol/liter hydrogen peroxide, with a limit of detection of 8.0 × 10^-9 mol/liter in a 10-min reaction period. It can easily be incorporated into the determination of biochemical substances that produce hydrogen peroxide under catalytic oxidation by their oxidase. This possibility has been tested for the determination of glucose in human sera as an example.

Original language	English
Pages (from-to)	332-338
Number of pages	7
Journal	Microchemical Journal
Volume	57
Issue number	3
DOIs	https://doi.org/10.1006/mchj.1997.1485
State	Published - Nov 1997

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title = "Application of o-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin",

abstract = "A highly sensitive fluorescence quenching method has been developed for selective determination of hydrogen peroxide based on the catalytic effect of hemin on the o-hydroxyphenylfluorone (a new fluorogenic substrate) and hydrogen peroxide system. Under optimum conditions, the calibration graph was linear over the range 0-1.0 × 10-6 mol/liter hydrogen peroxide, with a limit of detection of 8.0 × 10-9 mol/liter in a 10-min reaction period. It can easily be incorporated into the determination of biochemical substances that produce hydrogen peroxide under catalytic oxidation by their oxidase. This possibility has been tested for the determination of glucose in human sera as an example.",

author = "Zhu, \{Qing Zhi\} and Zheng, \{Xue Ying\} and Xu, \{Jin Gou\} and Liu, \{Feng Hua\} and Li, \{Qing Ge\}",

year = "1997",

month = nov,

doi = "10.1006/mchj.1997.1485",

language = "English",

volume = "57",

pages = "332--338",

journal = "Microchemical Journal",

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TY - JOUR

T1 - Application of o-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin

AU - Zhu, Qing Zhi

AU - Zheng, Xue Ying

AU - Xu, Jin Gou

AU - Liu, Feng Hua

AU - Li, Qing Ge

PY - 1997/11

Y1 - 1997/11

N2 - A highly sensitive fluorescence quenching method has been developed for selective determination of hydrogen peroxide based on the catalytic effect of hemin on the o-hydroxyphenylfluorone (a new fluorogenic substrate) and hydrogen peroxide system. Under optimum conditions, the calibration graph was linear over the range 0-1.0 × 10-6 mol/liter hydrogen peroxide, with a limit of detection of 8.0 × 10-9 mol/liter in a 10-min reaction period. It can easily be incorporated into the determination of biochemical substances that produce hydrogen peroxide under catalytic oxidation by their oxidase. This possibility has been tested for the determination of glucose in human sera as an example.

AB - A highly sensitive fluorescence quenching method has been developed for selective determination of hydrogen peroxide based on the catalytic effect of hemin on the o-hydroxyphenylfluorone (a new fluorogenic substrate) and hydrogen peroxide system. Under optimum conditions, the calibration graph was linear over the range 0-1.0 × 10-6 mol/liter hydrogen peroxide, with a limit of detection of 8.0 × 10-9 mol/liter in a 10-min reaction period. It can easily be incorporated into the determination of biochemical substances that produce hydrogen peroxide under catalytic oxidation by their oxidase. This possibility has been tested for the determination of glucose in human sera as an example.

UR - https://www.scopus.com/pages/publications/0001323926

U2 - 10.1006/mchj.1997.1485

DO - 10.1006/mchj.1997.1485

M3 - Article

AN - SCOPUS:0001323926

SN - 0026-265X

VL - 57

SP - 332

EP - 338

JO - Microchemical Journal

JF - Microchemical Journal

IS - 3

ER -

Application of o-Hydroxyphenylfluorone as a Fluorogenic Indicator to the Determination of Hydrogen Peroxide and Oxidase Substrates Based on the Catalytic Effect of Hemin

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