Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells

Brendan M. Heffernan; Stephanie A. Meyer; Diego Restrepo; Mark E. Siemens; Emily A. Gibson; Juliet T. Gopinath

doi:10.1364/CLEO_SI.2019.STu3H.3

Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells

Brendan M. Heffernan
, Stephanie A. Meyer
, Diego Restrepo
, Mark E. Siemens
, Emily A. Gibson
, Juliet T. Gopinath

Physiology and Biophysics

University of Colorado Boulder
University of Colorado Denver
University of Denver

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We demonstrate, for the first time, STED images of biological samples where fluorescence has been collected through the same fiber used to transport excitation and depletion light (through-fiber imaging). We also quantitatively demonstrate fiber-bending-independent resolution. These results show promise for endoscopic and in vivo super-resolution imaging.

Original language	English
Title of host publication	CLEO
Subtitle of host publication	Science and Innovations, CLEO_SI 2019
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Print)	9781943580576
DOIs	https://doi.org/10.1364/CLEO_SI.2019.STu3H.3
State	Published - 2019
Event	CLEO: Science and Innovations, CLEO_SI 2019 - San Jose, United States Duration: May 5 2019 → May 10 2019

Publication series

Name	Optics InfoBase Conference Papers
Volume	Part F129-CLEO_SI 2019
ISSN (Electronic)	2162-2701

Conference

Conference	CLEO: Science and Innovations, CLEO_SI 2019
Country/Territory	United States
City	San Jose
Period	05/5/19 → 05/10/19

Access to Document

10.1364/CLEO_SI.2019.STu3H.3

Cite this

Heffernan, B. M., Meyer, S. A., Restrepo, D., Siemens, M. E., Gibson, E. A., & Gopinath, J. T. (2019). Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells. In CLEO: Science and Innovations, CLEO_SI 2019 (Optics InfoBase Conference Papers; Vol. Part F129-CLEO_SI 2019). Optica Publishing Group (formerly OSA). https://doi.org/10.1364/CLEO_SI.2019.STu3H.3

@inproceedings{023b5bffc222440487d0db0adbc01362,

title = "Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells",

abstract = "We demonstrate, for the first time, STED images of biological samples where fluorescence has been collected through the same fiber used to transport excitation and depletion light (through-fiber imaging). We also quantitatively demonstrate fiber-bending-independent resolution. These results show promise for endoscopic and in vivo super-resolution imaging.",

author = "Heffernan, \{Brendan M.\} and Meyer, \{Stephanie A.\} and Diego Restrepo and Siemens, \{Mark E.\} and Gibson, \{Emily A.\} and Gopinath, \{Juliet T.\}",

note = "Publisher Copyright: {\textcopyright} 2019 The Author(s); CLEO: Science and Innovations, CLEO\_SI 2019 ; Conference date: 05-05-2019 Through 10-05-2019",

year = "2019",

doi = "10.1364/CLEO\_SI.2019.STu3H.3",

language = "English",

isbn = "9781943580576",

series = "Optics InfoBase Conference Papers",

publisher = "Optica Publishing Group (formerly OSA)",

booktitle = "CLEO",

}

Heffernan, BM, Meyer, SA, Restrepo, D, Siemens, ME, Gibson, EA & Gopinath, JT 2019, Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells. in CLEO: Science and Innovations, CLEO_SI 2019. Optics InfoBase Conference Papers, vol. Part F129-CLEO_SI 2019, Optica Publishing Group (formerly OSA), CLEO: Science and Innovations, CLEO_SI 2019, San Jose, United States, 05/5/19. https://doi.org/10.1364/CLEO_SI.2019.STu3H.3

Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells. / Heffernan, Brendan M.; Meyer, Stephanie A.; Restrepo, Diego et al.
CLEO: Science and Innovations, CLEO_SI 2019. Optica Publishing Group (formerly OSA), 2019. (Optics InfoBase Conference Papers; Vol. Part F129-CLEO_SI 2019).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells

AU - Heffernan, Brendan M.

AU - Meyer, Stephanie A.

AU - Restrepo, Diego

AU - Siemens, Mark E.

AU - Gibson, Emily A.

AU - Gopinath, Juliet T.

PY - 2019

Y1 - 2019

N2 - We demonstrate, for the first time, STED images of biological samples where fluorescence has been collected through the same fiber used to transport excitation and depletion light (through-fiber imaging). We also quantitatively demonstrate fiber-bending-independent resolution. These results show promise for endoscopic and in vivo super-resolution imaging.

AB - We demonstrate, for the first time, STED images of biological samples where fluorescence has been collected through the same fiber used to transport excitation and depletion light (through-fiber imaging). We also quantitatively demonstrate fiber-bending-independent resolution. These results show promise for endoscopic and in vivo super-resolution imaging.

UR - https://www.scopus.com/pages/publications/85068103389

U2 - 10.1364/CLEO_SI.2019.STu3H.3

DO - 10.1364/CLEO_SI.2019.STu3H.3

M3 - Conference contribution

AN - SCOPUS:85068103389

SN - 9781943580576

T3 - Optics InfoBase Conference Papers

BT - CLEO

PB - Optica Publishing Group (formerly OSA)

T2 - CLEO: Science and Innovations, CLEO_SI 2019

Y2 - 5 May 2019 through 10 May 2019

ER -

Bend-insensitive through-fiber stimulated emission depletion (STED) imaging of HeLa cells

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