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Cell-sensitive microscopy imaging for cell image segmentation

  • Shanghai Jiao Tong University
  • Stanford University
  • Missouri University of Science and Technology

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

10 Scopus citations

Abstract

We propose a novel cell segmentation approach by estimating a cell-sensitive camera response function based on variously exposed phase contrast microscopy images on the same cell dish. Using the cell-sensitive microscopy imaging, cells' original irradiance signals are restored from all exposures and the irradiance signals on non-cell background regions are restored as a uniform constant (i.e., the imaging system is sensitive to cells only but insensitive to non-cell background). Cell segmentation is then performed on the restored irradiance signals by simple thresholding. The experimental results validate that high quality cell segmentation can be achieved by our approach.

Original languageEnglish
Title of host publicationMedical Image Computing and Computer-Assisted Intervention, MICCAI 2014 - 17th International Conference, Proceedings
PublisherSpringer Verlag
Pages41-48
Number of pages8
EditionPART 1
ISBN (Print)9783319104034
DOIs
StatePublished - 2014
Event17th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2014 - Boston, MA, United States
Duration: Sep 14 2014Sep 18 2014

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
NumberPART 1
Volume8673 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference17th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2014
Country/TerritoryUnited States
CityBoston, MA
Period09/14/1409/18/14

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