TY - CHAP
T1 - Challenges in Detection of Antibiotics in Wastewater Matrix
AU - Mohapatra, Sanjeeb
AU - Padhye, Lokesh P.
AU - Mukherji, Suparna
N1 - Publisher Copyright:
© 2018, Springer Nature Singapore Pte Ltd.
PY - 2018
Y1 - 2018
N2 - Currently, there is increasing awareness regarding the effect of antibiotics on various organisms in the ecosystem even though they may occur at relatively low concentration. Thus, their concentration needs to be quantified precisely in various matrices including ground water, surface water, and municipal wastewater. The use of liquid chromatography together with high-resolution mass spectrometry (HR-LC-MS/MS) has enhanced the reliability, accessibility, and accuracy with which antibiotics can be detected. In the present study, a HR-LC-MS/MS-based method was developed for four antibiotics (ciprofloxacin, norfloxacin, azithromycin, and sulfamethoxazole). Initially, various mobile phases and LC columns were evaluated. The effect of solution pH, drying time of cartridge, and solvent used in the concentration step on recovery of antibiotics during solid-phase extraction (SPE) was also evaluated. Subsequently, the recovery of antibiotics from influent and effluent wastewater samples, obtained from a wastewater treatment plant (WWTP) in India, was determined based on the matrix spike method. The results revealed that for these wastewater samples even the use of structurally similar, isotopically labelled (deuterated) standards could not adequately improve the recovery of target antibiotics.
AB - Currently, there is increasing awareness regarding the effect of antibiotics on various organisms in the ecosystem even though they may occur at relatively low concentration. Thus, their concentration needs to be quantified precisely in various matrices including ground water, surface water, and municipal wastewater. The use of liquid chromatography together with high-resolution mass spectrometry (HR-LC-MS/MS) has enhanced the reliability, accessibility, and accuracy with which antibiotics can be detected. In the present study, a HR-LC-MS/MS-based method was developed for four antibiotics (ciprofloxacin, norfloxacin, azithromycin, and sulfamethoxazole). Initially, various mobile phases and LC columns were evaluated. The effect of solution pH, drying time of cartridge, and solvent used in the concentration step on recovery of antibiotics during solid-phase extraction (SPE) was also evaluated. Subsequently, the recovery of antibiotics from influent and effluent wastewater samples, obtained from a wastewater treatment plant (WWTP) in India, was determined based on the matrix spike method. The results revealed that for these wastewater samples even the use of structurally similar, isotopically labelled (deuterated) standards could not adequately improve the recovery of target antibiotics.
KW - Antibiotics
KW - Ion suppression
KW - Matrix effect
KW - Pharmaceuticals
KW - Solid-phase extraction (SPE)
UR - https://www.scopus.com/pages/publications/85064156468
U2 - 10.1007/978-981-10-7332-8_1
DO - 10.1007/978-981-10-7332-8_1
M3 - Chapter
AN - SCOPUS:85064156468
T3 - Energy, Environment, and Sustainability
SP - 3
EP - 20
BT - Energy, Environment, and Sustainability
PB - Springer Nature
ER -