Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis

A. B.F. Pacheco; K. C. Soares; D. F. De Almeida; G. I. Viboud; N. Binsztein; L. C.S. Ferreira

doi:10.1128/jcm.36.7.2099-2102.1998

Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis

A. B.F. Pacheco
, K. C. Soares
, D. F. De Almeida
, G. I. Viboud
, N. Binsztein
, L. C.S. Ferreira

Clinical Laboratory Sciences

Universidade Federal do Rio de Janeiro

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

Original language	English
Pages (from-to)	2099-2102
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	36
Issue number	7
DOIs	https://doi.org/10.1128/jcm.36.7.2099-2102.1998
State	Published - 1998

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10.1128/jcm.36.7.2099-2102.1998

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@article{36735e301bc24384907579d0a8ef9bc5,

title = "Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis",

abstract = "The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82\% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.",

author = "Pacheco, \{A. B.F.\} and Soares, \{K. C.\} and \{De Almeida\}, \{D. F.\} and Viboud, \{G. I.\} and N. Binsztein and Ferreira, \{L. C.S.\}",

year = "1998",

doi = "10.1128/jcm.36.7.2099-2102.1998",

language = "English",

volume = "36",

pages = "2099--2102",

journal = "Journal of Clinical Microbiology",

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T1 - Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis

AU - Pacheco, A. B.F.

AU - Soares, K. C.

AU - De Almeida, D. F.

AU - Viboud, G. I.

AU - Binsztein, N.

AU - Ferreira, L. C.S.

PY - 1998

Y1 - 1998

N2 - The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

AB - The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

UR - https://www.scopus.com/pages/publications/0031838457

U2 - 10.1128/jcm.36.7.2099-2102.1998

DO - 10.1128/jcm.36.7.2099-2102.1998

M3 - Article

C2 - 9650973

AN - SCOPUS:0031838457

SN - 0095-1137

VL - 36

SP - 2099

EP - 2102

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis

Abstract

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