Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina

I. Ishimoto; H. Kiyama; K. Hamano; S. Shiosaka; C. C. Malbon; M. Nakauchi; P. C. Emson; R. Manabe; M. Tohyama

doi:10.1016/0168-0102(89)90020-5

Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina

I. Ishimoto
, H. Kiyama
, K. Hamano
, S. Shiosaka
, C. C. Malbon
, M. Nakauchi
, P. C. Emson
, R. Manabe
, M. Tohyama

Pharmacological Sciences

The University of Osaka
Babraham Institute

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Using antisera against tyrosine hydroxylase (TH) and purified β₂-adrenergic receptors (β₂-AdR), we found that TH- and AdR-like immunoreactivities coexisted in large amacrine cells. These findings indicated an association between dopamine-containing amacrine cells and adrenergic amacrine cells. The present study also showed that amacrine cells with TH-like immunoreactivity have vitamin-D-dependent calcium-binding protein (calbindin, 27 000 kDa)-like immunoreactivity as well, suggesting that calbindin plays an important postsynaptic role in dopaminergic amacrine cells.

Original language	English
Pages (from-to)	257-263
Number of pages	7
Journal	Neuroscience Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/0168-0102(89)90020-5
State	Published - Dec 1989

Keywords

Adrenergic receptors
Calbindin
Co-localization
Dopamine
Immunocytochemistry
Rat retina
Tyrosine hydroxylase

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10.1016/0168-0102(89)90020-5

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title = "Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina",

abstract = "Using antisera against tyrosine hydroxylase (TH) and purified β2-adrenergic receptors (β2-AdR), we found that TH- and AdR-like immunoreactivities coexisted in large amacrine cells. These findings indicated an association between dopamine-containing amacrine cells and adrenergic amacrine cells. The present study also showed that amacrine cells with TH-like immunoreactivity have vitamin-D-dependent calcium-binding protein (calbindin, 27 000 kDa)-like immunoreactivity as well, suggesting that calbindin plays an important postsynaptic role in dopaminergic amacrine cells.",

keywords = "Adrenergic receptors, Calbindin, Co-localization, Dopamine, Immunocytochemistry, Rat retina, Tyrosine hydroxylase",

author = "I. Ishimoto and H. Kiyama and K. Hamano and S. Shiosaka and Malbon, \{C. C.\} and M. Nakauchi and Emson, \{P. C.\} and R. Manabe and M. Tohyama",

year = "1989",

month = dec,

doi = "10.1016/0168-0102(89)90020-5",

language = "English",

volume = "7",

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journal = "Neuroscience Research",

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TY - JOUR

T1 - Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina

AU - Ishimoto, I.

AU - Kiyama, H.

AU - Hamano, K.

AU - Shiosaka, S.

AU - Malbon, C. C.

AU - Nakauchi, M.

AU - Emson, P. C.

AU - Manabe, R.

AU - Tohyama, M.

PY - 1989/12

Y1 - 1989/12

N2 - Using antisera against tyrosine hydroxylase (TH) and purified β2-adrenergic receptors (β2-AdR), we found that TH- and AdR-like immunoreactivities coexisted in large amacrine cells. These findings indicated an association between dopamine-containing amacrine cells and adrenergic amacrine cells. The present study also showed that amacrine cells with TH-like immunoreactivity have vitamin-D-dependent calcium-binding protein (calbindin, 27 000 kDa)-like immunoreactivity as well, suggesting that calbindin plays an important postsynaptic role in dopaminergic amacrine cells.

AB - Using antisera against tyrosine hydroxylase (TH) and purified β2-adrenergic receptors (β2-AdR), we found that TH- and AdR-like immunoreactivities coexisted in large amacrine cells. These findings indicated an association between dopamine-containing amacrine cells and adrenergic amacrine cells. The present study also showed that amacrine cells with TH-like immunoreactivity have vitamin-D-dependent calcium-binding protein (calbindin, 27 000 kDa)-like immunoreactivity as well, suggesting that calbindin plays an important postsynaptic role in dopaminergic amacrine cells.

KW - Adrenergic receptors

KW - Calbindin

KW - Co-localization

KW - Dopamine

KW - Immunocytochemistry

KW - Rat retina

KW - Tyrosine hydroxylase

UR - https://www.scopus.com/pages/publications/0024789589

U2 - 10.1016/0168-0102(89)90020-5

DO - 10.1016/0168-0102(89)90020-5

M3 - Article

C2 - 2575732

AN - SCOPUS:0024789589

SN - 0168-0102

VL - 7

SP - 257

EP - 263

JO - Neuroscience Research

JF - Neuroscience Research

IS - 3

ER -

Co-localization of adrenergic receptors and vitamin-D-dependent calcium-binding protein (calbindin) in the dopaminergic amacrine cells of the rat retina

Abstract

Keywords

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