Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

D. E. Shevell; A. M. Abou-Zamzam; B. Demple; G. C. Walker

doi:10.1128/jb.170.7.3294-3296.1988

Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

D. E. Shevell
, A. M. Abou-Zamzam
, B. Demple
, G. C. Walker

Pharmacological Sciences

Massachusetts Institute of Technology

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

Original language	English
Pages (from-to)	3294-3296
Number of pages	3
Journal	Journal of Bacteriology
Volume	170
Issue number	7
DOIs	https://doi.org/10.1128/jb.170.7.3294-3296.1988
State	Published - Jul 1988

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10.1128/jb.170.7.3294-3296.1988

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title = "Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.",

abstract = "We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.",

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AU - Shevell, D. E.

AU - Abou-Zamzam, A. M.

AU - Demple, B.

AU - Walker, G. C.

PY - 1988/7

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N2 - We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

AB - We constructed an ada deletion by gene replacement in a recD1014 strain of Escherichia coli. Characterization of a delta ada-25 recD+ strain revealed the presence of a second DNA methyltransferase activity in E. coli K-12 which transfers a methyl group from methylated DNA to a protein with a molecular weight of 18,000 to 20,000.

UR - https://www.scopus.com/pages/publications/0024041533

U2 - 10.1128/jb.170.7.3294-3296.1988

DO - 10.1128/jb.170.7.3294-3296.1988

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C2 - 3290202

AN - SCOPUS:0024041533

SN - 0021-9193

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JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 7

ER -

Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA.

Abstract

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