Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

Ming Rui Duan; Hui Ren; Peng Mao; Chun Hong Wei; Yu He Liang; Yi Li; Xiao Dong Su

doi:10.1016/j.bbapap.2005.03.013

Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

Ming Rui Duan
, Hui Ren
, Peng Mao
, Chun Hong Wei
, Yu He Liang
, Yi Li
, Xiao Dong Su

Pathology

Peking University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 Å resolution in-house and belongs to space group P2₁ with unit-cell parameters a = 64.10 Å, b = 34.88 Å, c = 114.72 Å, β = 90.49°.

Original language	English
Pages (from-to)	14-16
Number of pages	3
Journal	Biochimica et Biophysica Acta - Proteins and Proteomics
Volume	1750
Issue number	1
DOIs	https://doi.org/10.1016/j.bbapap.2005.03.013
State	Published - Jun 15 2005

Keywords

Crystallization
Transcription factor
WRKY protein
X-ray diffraction

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10.1016/j.bbapap.2005.03.013

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title = "Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor",

abstract = "The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 {\AA} resolution in-house and belongs to space group P21 with unit-cell parameters a = 64.10 {\AA}, b = 34.88 {\AA}, c = 114.72 {\AA}, β = 90.49°.",

keywords = "Crystallization, Transcription factor, WRKY protein, X-ray diffraction",

author = "Duan, \{Ming Rui\} and Hui Ren and Peng Mao and Wei, \{Chun Hong\} and Liang, \{Yu He\} and Yi Li and Su, \{Xiao Dong\}",

year = "2005",

month = jun,

day = "15",

doi = "10.1016/j.bbapap.2005.03.013",

language = "English",

volume = "1750",

pages = "14--16",

journal = "Biochimica et Biophysica Acta - Proteins and Proteomics",

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T1 - Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

AU - Duan, Ming Rui

AU - Ren, Hui

AU - Mao, Peng

AU - Wei, Chun Hong

AU - Liang, Yu He

AU - Li, Yi

AU - Su, Xiao Dong

PY - 2005/6/15

Y1 - 2005/6/15

N2 - The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 Å resolution in-house and belongs to space group P21 with unit-cell parameters a = 64.10 Å, b = 34.88 Å, c = 114.72 Å, β = 90.49°.

AB - The C-terminal WRKY domain of Arabidopsis thaliana WRKY1 protein, a transcription factor, was cloned and expressed. The expressed protein was then purified and crystallized. The preliminary X-ray analysis was undertaken. The crystal diffracted to 2.50 Å resolution in-house and belongs to space group P21 with unit-cell parameters a = 64.10 Å, b = 34.88 Å, c = 114.72 Å, β = 90.49°.

KW - Crystallization

KW - Transcription factor

KW - WRKY protein

KW - X-ray diffraction

UR - https://www.scopus.com/pages/publications/20444367355

U2 - 10.1016/j.bbapap.2005.03.013

DO - 10.1016/j.bbapap.2005.03.013

M3 - Article

C2 - 15935985

AN - SCOPUS:20444367355

SN - 1570-9639

VL - 1750

SP - 14

EP - 16

JO - Biochimica et Biophysica Acta - Proteins and Proteomics

JF - Biochimica et Biophysica Acta - Proteins and Proteomics

IS - 1

ER -

Crystallization and preliminary X-ray analysis of the C-terminal WRKY domain of Arabidopsis thaliana WRKY1 transcription factor

Abstract

Keywords

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