Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR

Josephine Wu; Wandi Zhang; Boxun Xie; Maoxin Wu; Xiaodi Tong; Jayant Kalpoe; David Zhang

doi:10.1128/JCM.01324-08

Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR

Josephine Wu
, Wandi Zhang
, Boxun Xie
, Maoxin Wu
, Xiaodi Tong
, Jayant Kalpoe
, David Zhang

Pathology

Icahn School of Medicine at Mount Sinai
Leiden University

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

Original language	English
Pages (from-to)	807-810
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	47
Issue number	3
DOIs	https://doi.org/10.1128/JCM.01324-08
State	Published - Mar 2009

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title = "Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR",

abstract = "Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.",

author = "Josephine Wu and Wandi Zhang and Boxun Xie and Maoxin Wu and Xiaodi Tong and Jayant Kalpoe and David Zhang",

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AU - Wu, Josephine

AU - Zhang, Wandi

AU - Xie, Boxun

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AU - Tong, Xiaodi

AU - Kalpoe, Jayant

AU - Zhang, David

PY - 2009/3

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N2 - Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

AB - Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

UR - https://www.scopus.com/pages/publications/62749179691

U2 - 10.1128/JCM.01324-08

DO - 10.1128/JCM.01324-08

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SN - 0095-1137

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JO - Journal of Clinical Microbiology

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ER -

Detection and toxin typing of Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples by PCR

Abstract

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