Determination of ceramides and diglycerides by the diglyceride kinase assay

Alicja Bielawska; David K. Perry; Yusuf A. Hannun

doi:10.1006/abio.2001.5342

Determination of ceramides and diglycerides by the diglyceride kinase assay

Alicja Bielawska
, David K. Perry
, Yusuf A. Hannun

Cancer Center

Medical University of South Carolina

Research output: Contribution to journal › Review article › peer-review

80 Scopus citations

Abstract

This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods.

Original language	English
Pages (from-to)	141-150
Number of pages	10
Journal	Analytical Biochemistry
Volume	298
Issue number	2
DOIs	https://doi.org/10.1006/abio.2001.5342
State	Published - Dec 15 2001

Keywords

Ceramides
DG kinase
Diglycerides

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10.1006/abio.2001.5342

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title = "Determination of ceramides and diglycerides by the diglyceride kinase assay",

abstract = "This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods.",

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T1 - Determination of ceramides and diglycerides by the diglyceride kinase assay

AU - Bielawska, Alicja

AU - Perry, David K.

AU - Hannun, Yusuf A.

PY - 2001/12/15

Y1 - 2001/12/15

N2 - This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods.

AB - This review will discuss the utilization of the diglyceride (DG) kinase assay as an analytical method that allows the simultaneous quantitation of DG and ceramide from cell and tissue samples. This enzymatic approach is sensitive, quantitative, and linear over a broad range (20 pmol to 20-25 nmol) for both classes of lipids. It is also practical in that it can be applied to crude lipid extracts and used to process many samples (up to 100) concurrently. However, it has become apparent that this assay has not been conducted optimally, primarily as a result of lack of adherence to its basic principles. The principles illustrated here are also useful to all enzymatic quantitative methods.

KW - Ceramides

KW - DG kinase

KW - Diglycerides

UR - https://www.scopus.com/pages/publications/0035892633

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DO - 10.1006/abio.2001.5342

M3 - Review article

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SN - 0003-2697

VL - 298

SP - 141

EP - 150

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Determination of ceramides and diglycerides by the diglyceride kinase assay

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Keywords

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