Determination of glucose based on the effect of photons as a substitute for glucose oxidase

Glucose can produce H₂O₂ in solution when irradiated with UV light even in the absence of glucose oxidase. Based on this effect, photons were utilized as a substrate for glucose oxidase, and a new fluorescent method was developed for indirect determination of glucose by a coupled fluorescence reaction of p-hydroxyphenyl acetic acid and H₂O₂ with hemin as a catalyst. The calibration graph is linear over the range 0-6.0x10^-7mol/l glucose, the detection limit is 1.7x10^-8mol/l glucose. The method has been applied to the determination of glucose in human sera with satisfactory results. Copyright (C) 1999 Elsevier Science B.V.