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Deterministic and true single-cell encapsulation

  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present a novel method to perform highly efficient true single-cell encapsulation where we trade off the traditional throughput of droplet microfluidics for higher control of operation. We developed a unique approach that consists in a trapping-encapsulation sequence that encapsulates and retrieves most cells injected into the device. Our strategy is passive and efficiently encapsulates hundreds of individual cells into droplets without sample loss, effectively laying the foundations for the single-cell analysis of rare samples via droplet microfluidics.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages947-948
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Cell isolation
  • Droplets
  • Encapsulation
  • Single cell analysis

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