Directing flux in glycan biosynthetic pathways with a small molecule switch

Jennifer J. Kohler; Jennifer L. Czlapinski; Scott T. Laughlin; Michael W. Schelle; Christopher L. De Graffenried; Carolyn R. Bertozzi

doi:10.1002/cbic.200400156

Directing flux in glycan biosynthetic pathways with a small molecule switch

Jennifer J. Kohler
, Jennifer L. Czlapinski
, Scott T. Laughlin
, Michael W. Schelle
, Christopher L. De Graffenried
, Carolyn R. Bertozzi

Chemistry

University of California at Berkeley
Yale University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Directing sugar traffic. Golgi-resident glycosyltransferases act in concert to direct synthesis of a wide variety of cell-surface glycans. A re-engineered fucosyltransferase, whose activity is controlled with the small molecule rapamycin, can be used to divert flux away from production of one glycan (αGal) and toward synthesis of another (H antigen).

Original language	English
Pages (from-to)	1455-1458
Number of pages	4
Journal	ChemBioChem
Volume	5
Issue number	10
DOIs	https://doi.org/10.1002/cbic.200400156
State	Published - Oct 4 2004

Keywords

Carbohydrates
Cell surface
Chemical genetics
Glycosylation
Protein engineering

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10.1002/cbic.200400156

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title = "Directing flux in glycan biosynthetic pathways with a small molecule switch",

abstract = "Directing sugar traffic. Golgi-resident glycosyltransferases act in concert to direct synthesis of a wide variety of cell-surface glycans. A re-engineered fucosyltransferase, whose activity is controlled with the small molecule rapamycin, can be used to divert flux away from production of one glycan (αGal) and toward synthesis of another (H antigen).",

keywords = "Carbohydrates, Cell surface, Chemical genetics, Glycosylation, Protein engineering",

author = "Kohler, \{Jennifer J.\} and Czlapinski, \{Jennifer L.\} and Laughlin, \{Scott T.\} and Schelle, \{Michael W.\} and \{De Graffenried\}, \{Christopher L.\} and Bertozzi, \{Carolyn R.\}",

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T1 - Directing flux in glycan biosynthetic pathways with a small molecule switch

AU - Kohler, Jennifer J.

AU - Czlapinski, Jennifer L.

AU - Laughlin, Scott T.

AU - Schelle, Michael W.

AU - De Graffenried, Christopher L.

AU - Bertozzi, Carolyn R.

PY - 2004/10/4

Y1 - 2004/10/4

N2 - Directing sugar traffic. Golgi-resident glycosyltransferases act in concert to direct synthesis of a wide variety of cell-surface glycans. A re-engineered fucosyltransferase, whose activity is controlled with the small molecule rapamycin, can be used to divert flux away from production of one glycan (αGal) and toward synthesis of another (H antigen).

AB - Directing sugar traffic. Golgi-resident glycosyltransferases act in concert to direct synthesis of a wide variety of cell-surface glycans. A re-engineered fucosyltransferase, whose activity is controlled with the small molecule rapamycin, can be used to divert flux away from production of one glycan (αGal) and toward synthesis of another (H antigen).

KW - Carbohydrates

KW - Cell surface

KW - Chemical genetics

KW - Glycosylation

KW - Protein engineering

UR - https://www.scopus.com/pages/publications/5644236683

U2 - 10.1002/cbic.200400156

DO - 10.1002/cbic.200400156

M3 - Article

C2 - 15457531

AN - SCOPUS:5644236683

SN - 1439-4227

VL - 5

SP - 1455

EP - 1458

JO - ChemBioChem

JF - ChemBioChem

IS - 10

ER -

Directing flux in glycan biosynthetic pathways with a small molecule switch

Abstract

Keywords

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