Droplet-based high-throughput live/dead cell assay

Eric Brouzes; Jeffrey Branciforte; Mariusz Twardowski; Dave Marran; Yue Suo; Yves Charles; Laurent Boitard; Michael P. Weiner; Jonathan M. Rothberg; Norbert Perrimon; Darren R. Link

Droplet-based high-throughput live/dead cell assay

Eric Brouzes
, Jeffrey Branciforte
, Mariusz Twardowski
, Dave Marran
, Yue Suo
, Yves Charles
, Laurent Boitard
, Michael P. Weiner
, Jonathan M. Rothberg
, Norbert Perrimon
, Darren R. Link

Biomedical Engineering

RainDance Technologies
Harvard University
Howard Hughes Medical Institute

Research output: Contribution to conference › Paper › peer-review

5 Scopus citations

Abstract

We have developed an integrated live/dead assay for scoring the viability of cells encapsulated within droplets on a microfluidic device [1]. The device allows us to fuse [2] cell-containing droplets with dye-containing droplets. The combining, mixing [3], incubation and detection of cell viability are performed on-chip.

Original language	English
Pages	1043-1045
Number of pages	3
State	Published - 2006
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

Keywords

Cells
Droplets
Live/dead assay

Cite this

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title = "Droplet-based high-throughput live/dead cell assay",

abstract = "We have developed an integrated live/dead assay for scoring the viability of cells encapsulated within droplets on a microfluidic device [1]. The device allows us to fuse [2] cell-containing droplets with dye-containing droplets. The combining, mixing [3], incubation and detection of cell viability are performed on-chip.",

keywords = "Cells, Droplets, Live/dead assay",

author = "Eric Brouzes and Jeffrey Branciforte and Mariusz Twardowski and Dave Marran and Yue Suo and Yves Charles and Laurent Boitard and Weiner, \{Michael P.\} and Rothberg, \{Jonathan M.\} and Norbert Perrimon and Link, \{Darren R.\}",

year = "2006",

language = "English",

pages = "1043--1045",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

}

TY - CONF

T1 - Droplet-based high-throughput live/dead cell assay

AU - Brouzes, Eric

AU - Branciforte, Jeffrey

AU - Twardowski, Mariusz

AU - Marran, Dave

AU - Suo, Yue

AU - Charles, Yves

AU - Boitard, Laurent

AU - Weiner, Michael P.

AU - Rothberg, Jonathan M.

AU - Perrimon, Norbert

AU - Link, Darren R.

PY - 2006

Y1 - 2006

N2 - We have developed an integrated live/dead assay for scoring the viability of cells encapsulated within droplets on a microfluidic device [1]. The device allows us to fuse [2] cell-containing droplets with dye-containing droplets. The combining, mixing [3], incubation and detection of cell viability are performed on-chip.

AB - We have developed an integrated live/dead assay for scoring the viability of cells encapsulated within droplets on a microfluidic device [1]. The device allows us to fuse [2] cell-containing droplets with dye-containing droplets. The combining, mixing [3], incubation and detection of cell viability are performed on-chip.

KW - Cells

KW - Droplets

KW - Live/dead assay

UR - https://www.scopus.com/pages/publications/51049112388

M3 - Paper

AN - SCOPUS:51049112388

SP - 1043

EP - 1045

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Droplet-based high-throughput live/dead cell assay

Abstract

Conference

Keywords

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Cite this