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Dynamic light-scattering studies of BSA and lysozyme using a backscatter fiber optic system

  • Stony Brook University

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

6 Scopus citations

Abstract

A comparative dynamic light scattering study of BSA and lysozyme is presented. A backscatter fiber optic system and a conventional light scattering spectrometer are used to measure the diameter of proteins in the dilute regime, that is, below concentrations of 10 mg/ml. The fiber optic system operating with a power level of 2 mW at a wavelength of 632.8 nm compares favorably with a conventional system operating with a power level of 40 mW at a wavelength of 514.5 nm. Quasi-elastic light scattering measurements taken at several concentrations demonstrate the utility of a backscatter fiber optic probe for sizing of small molecular weight proteins. The fiber probe, comprising two optical fibers, is about 3 mm in diameter and can be positioned either inside or outside the scattering cell.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Society of Photo-Optical Instrumentation Engineers
Pages16-24
Number of pages9
ISBN (Print)0819411116, 9780819411112
DOIs
StatePublished - 1993
EventStatic and Dynamic Light Scattering in Medicine and Biology - Los Angeles, CA, USA
Duration: Jan 21 1993Jan 22 1993

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1884
ISSN (Print)0277-786X

Conference

ConferenceStatic and Dynamic Light Scattering in Medicine and Biology
CityLos Angeles, CA, USA
Period01/21/9301/22/93

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