Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting

Dmitri N. Gavrilov; Boris Gorbovitski; Mikhail Gouzman; Georgiy Gudkov; Andrei Stepoukhovitch; Victor Ruskovoloshin; Andrei Tsuprik; Georgiy Tyshko; Olga Bilenko; Olga Kosobokova; Serge Luryi; Vera Gorfinkel

doi:10.1002/elps.200390152

Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting

Dmitri N. Gavrilov
, Boris Gorbovitski
, Mikhail Gouzman
, Georgiy Gudkov
, Andrei Stepoukhovitch
, Victor Ruskovoloshin
, Andrei Tsuprik
, Georgiy Tyshko
, Olga Bilenko
, Olga Kosobokova
, Serge Luryi
, Vera Gorfinkel

Electrical Engineering

Stony Brook University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.

Original language	English
Pages (from-to)	1184-1192
Number of pages	9
Journal	Electrophoresis
Volume	24
Issue number	7-8
DOIs	https://doi.org/10.1002/elps.200390152
State	Published - Apr 2003

Keywords

DNA sequencing instruments
Single-photon detection

Access to Document

10.1002/elps.200390152

Cite this

Gavrilov, D. N., Gorbovitski, B., Gouzman, M., Gudkov, G., Stepoukhovitch, A., Ruskovoloshin, V., Tsuprik, A., Tyshko, G., Bilenko, O., Kosobokova, O., Luryi, S., & Gorfinkel, V. (2003). Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting. Electrophoresis, 24(7-8), 1184-1192. https://doi.org/10.1002/elps.200390152

@article{c2f808ef5d0e4c1abe572a3192745d3a,

title = "Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting",

abstract = "Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.",

keywords = "DNA sequencing instruments, Single-photon detection",

author = "Gavrilov, \{Dmitri N.\} and Boris Gorbovitski and Mikhail Gouzman and Georgiy Gudkov and Andrei Stepoukhovitch and Victor Ruskovoloshin and Andrei Tsuprik and Georgiy Tyshko and Olga Bilenko and Olga Kosobokova and Serge Luryi and Vera Gorfinkel",

year = "2003",

month = apr,

doi = "10.1002/elps.200390152",

language = "English",

volume = "24",

pages = "1184--1192",

journal = "Electrophoresis",

issn = "0173-0835",

number = "7-8",

}

Gavrilov, DN, Gorbovitski, B, Gouzman, M, Gudkov, G, Stepoukhovitch, A, Ruskovoloshin, V, Tsuprik, A, Tyshko, G, Bilenko, O, Kosobokova, O, Luryi, S & Gorfinkel, V 2003, 'Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting', Electrophoresis, vol. 24, no. 7-8, pp. 1184-1192. https://doi.org/10.1002/elps.200390152

TY - JOUR

T1 - Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting

AU - Gavrilov, Dmitri N.

AU - Gorbovitski, Boris

AU - Gouzman, Mikhail

AU - Gudkov, Georgiy

AU - Stepoukhovitch, Andrei

AU - Ruskovoloshin, Victor

AU - Tsuprik, Andrei

AU - Tyshko, Georgiy

AU - Bilenko, Olga

AU - Kosobokova, Olga

AU - Luryi, Serge

AU - Gorfinkel, Vera

PY - 2003/4

Y1 - 2003/4

N2 - Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.

AB - Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.

KW - DNA sequencing instruments

KW - Single-photon detection

UR - https://www.scopus.com/pages/publications/9144260562

U2 - 10.1002/elps.200390152

DO - 10.1002/elps.200390152

M3 - Article

C2 - 12707910

AN - SCOPUS:9144260562

SN - 0173-0835

VL - 24

SP - 1184

EP - 1192

JO - Electrophoresis

JF - Electrophoresis

IS - 7-8

ER -

Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this