Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127

Julie E. Matthew; Yesenia L. Nazario; Susan C. Roberts; Surita R. Bhatia

doi:10.1016/S0142-9612(02)00208-9

Effect of mammalian cell culture medium on the gelation properties of Pluronic^® F127

Julie E. Matthew
, Yesenia L. Nazario
, Susan C. Roberts
, Surita R. Bhatia

Chemistry

University of Massachusetts

Research output: Contribution to journal › Article › peer-review

114 Scopus citations

Abstract

We investigate the gelation of a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) copolymer, Pluronic^® F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics^® and applications where copolymer concentration must be minimized due to toxicity concerns.

Original language	English
Pages (from-to)	4615-4619
Number of pages	5
Journal	Biomaterials
Volume	23
Issue number	23
DOIs	https://doi.org/10.1016/S0142-9612(02)00208-9
State	Published - Dec 2002

Keywords

Cell culture
Encapsulation
PEO-PPO-PEO
Sol-gel boundary
Triblock copolymers

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10.1016/S0142-9612(02)00208-9

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title = "Effect of mammalian cell culture medium on the gelation properties of Pluronic{\textregistered} F127",

abstract = "We investigate the gelation of a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) copolymer, Pluronic{\textregistered} F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics{\textregistered} and applications where copolymer concentration must be minimized due to toxicity concerns.",

keywords = "Cell culture, Encapsulation, PEO-PPO-PEO, Sol-gel boundary, Triblock copolymers",

author = "Matthew, \{Julie E.\} and Nazario, \{Yesenia L.\} and Roberts, \{Susan C.\} and Bhatia, \{Surita R.\}",

year = "2002",

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TY - JOUR

T1 - Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127

AU - Matthew, Julie E.

AU - Nazario, Yesenia L.

AU - Roberts, Susan C.

AU - Bhatia, Surita R.

PY - 2002/12

Y1 - 2002/12

N2 - We investigate the gelation of a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) copolymer, Pluronic® F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics® and applications where copolymer concentration must be minimized due to toxicity concerns.

AB - We investigate the gelation of a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) copolymer, Pluronic® F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics® and applications where copolymer concentration must be minimized due to toxicity concerns.

KW - Cell culture

KW - Encapsulation

KW - PEO-PPO-PEO

KW - Sol-gel boundary

KW - Triblock copolymers

UR - https://www.scopus.com/pages/publications/0036971305

U2 - 10.1016/S0142-9612(02)00208-9

DO - 10.1016/S0142-9612(02)00208-9

M3 - Article

C2 - 12322983

AN - SCOPUS:0036971305

SN - 0142-9612

VL - 23

SP - 4615

EP - 4619

JO - Biomaterials

JF - Biomaterials

IS - 23

ER -

Effect of mammalian cell culture medium on the gelation properties of Pluronic^® F127

Abstract

Keywords

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