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Efficacy assessment of hyaluronan vehicles for sustained release of pDNA

  • Weiliam Chen
  • , Angela Kim
  • , Mary Frame
  • , Daniel Checkla
  • , Yang Hyun Yun
  • , Paige Yellen
  • , Philip Dehazya
  • Stony Brook University
  • Clear Solutions, Inc.

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Natural polymers have many advantages over their synthetic counterparts for biomedical uses; carbohydrate is a class of under-explored natural polymers for gene delivery. We have formulated microspheres and wafer matrices from Hyaluronan (HA) for delivery of plasmid DNA (pDNA). Sustained releases of pDNA over a period of at least 1 month were achieved in both formulations. Results from cell culture transfection studies indicated that the pDNA released from these systems were both intact and bioactive. Microsphere formulations for sustained delivery of pDNA encoding Vascular Endothelial Growth Factor (VEGF) were infiltrated into adult hamster cheek pouches, the microvascular responses differed from control. Wafer matrix formulations for sustained release of pDNA encoding Platelet Derived Growth Factor-BB (PDGFBB) were implanted in pig full-thickness dermal wound models and the results indicated acceleration of wound repair.

Original languageEnglish
Title of host publicationNew Delivery Systems for Controlled Drug Release from Naturally Occurring Materials
PublisherAmerican Chemical Society
Pages3-16
Number of pages14
ISBN (Print)9780841274242
DOIs
StatePublished - Nov 7 2008

Publication series

NameACS Symposium Series
Volume992
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

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