Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

B. Liu; Z. Nie; Y. G. Song; Y. P. Liu; K. J. Liu; Q. Tian; Yang Liu

doi:10.1007/BF03166336

Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

B. Liu
, Z. Nie
, Y. G. Song
, Y. P. Liu
, K. J. Liu
, Q. Tian
, Yang Liu

Pathology

CAS - Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-1- pyrroline-1-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylene-triaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K₃Fe(CN)₆ and CuSO₄. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO by activated charcoal treatment.

Original language	English
Pages (from-to)	597-604
Number of pages	8
Journal	Applied Magnetic Resonance
Volume	29
Issue number	4
DOIs	https://doi.org/10.1007/BF03166336
State	Published - 2005

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title = "Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments",

abstract = "There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-1- pyrroline-1-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylene-triaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K3Fe(CN)6 and CuSO4. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO by activated charcoal treatment.",

author = "B. Liu and Z. Nie and Song, \{Y. G.\} and Liu, \{Y. P.\} and Liu, \{K. J.\} and Q. Tian and Yang Liu",

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TY - JOUR

T1 - Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

AU - Liu, B.

AU - Nie, Z.

AU - Song, Y. G.

AU - Liu, Y. P.

AU - Liu, K. J.

AU - Tian, Q.

AU - Liu, Yang

PY - 2005

Y1 - 2005

N2 - There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-1- pyrroline-1-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylene-triaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K3Fe(CN)6 and CuSO4. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO by activated charcoal treatment.

AB - There exists hydroxylamine impurity in 5-(diethoxyphosphoryl)-5-methyl-1- pyrroline-1-oxide (DEPMPO) despite the purification by column separation and molecular distillation. Slow oxidation of hydroxylamine impurity to nitroxide is caused by a trace amount of transition metal ions in the solution instead by oxygen, and it can be conveniently inhibited upon the addition of diethylene-triaminepentaacetic acid, a transition metal ion chelator. Hydroxylamine impurity in DEPMPO can also be oxidized to electron spin resonance detectable paramagnetic nitroxide in the presence of mild oxidants, such as K3Fe(CN)6 and CuSO4. The previously recommended procedure for elimination of hydroxylamines was further simplified in the present study. Both hydroxylamine and nitroxide impurities can be removed from the aqueous solution of DEPMPO by activated charcoal treatment.

UR - https://www.scopus.com/pages/publications/33646536547

U2 - 10.1007/BF03166336

DO - 10.1007/BF03166336

M3 - Article

AN - SCOPUS:33646536547

SN - 0937-9347

VL - 29

SP - 597

EP - 604

JO - Applied Magnetic Resonance

JF - Applied Magnetic Resonance

IS - 4

ER -

Eliminating and inhibiting hydroxylamine oxidation in DEPMPO spin trapping experiments

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