Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Jinzhu Yu; Ki Oh; Sitapriya Moorthi; Ling Li; Helmut H. Strey; Michael Schuster; Chiara Luberto; Phenix Lan Quan; Eric Brouzes

doi:10.1039/c9lc01213c

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Jinzhu Yu
, Ki Oh
, Sitapriya Moorthi
, Ling Li
, Helmut H. Strey
, Michael Schuster
, Chiara Luberto
, Phenix Lan Quan
, Eric Brouzes

Stony Brook University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

Original language	English
Pages (from-to)	942-948
Number of pages	7
Journal	Lab on a Chip
Volume	20
Issue number	5
DOIs	https://doi.org/10.1039/c9lc01213c
State	Published - Mar 7 2020

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10.1039/c9lc01213c

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title = "Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML",

abstract = "We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.",

author = "Jinzhu Yu and Ki Oh and Sitapriya Moorthi and Ling Li and Strey, \{Helmut H.\} and Michael Schuster and Chiara Luberto and Quan, \{Phenix Lan\} and Eric Brouzes",

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T1 - Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

AU - Yu, Jinzhu

AU - Oh, Ki

AU - Moorthi, Sitapriya

AU - Li, Ling

AU - Strey, Helmut H.

AU - Schuster, Michael

AU - Luberto, Chiara

AU - Quan, Phenix Lan

AU - Brouzes, Eric

PY - 2020/3/7

Y1 - 2020/3/7

N2 - We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

AB - We developed a simple, rapid and cost-effective enzymatic-based cytometry platform to measure intracellular signaling pathway activity. Our single-cell microwell array platform quantifies protein phosphorylation using enzymatic signal amplification and exploiting Michaelis-Menten kinetics. Our method provides a two-fold increase in resolution compared to conventional flow cytometry.

UR - https://www.scopus.com/pages/publications/85081125957

U2 - 10.1039/c9lc01213c

DO - 10.1039/c9lc01213c

M3 - Article

C2 - 32031548

AN - SCOPUS:85081125957

SN - 1473-0197

VL - 20

SP - 942

EP - 948

JO - Lab on a Chip

JF - Lab on a Chip

IS - 5

ER -

Enzymatic-based cytometry, a sensitive single-cell cytometric method to assess BCR-ABL1 activity in CML

Abstract

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