Enzymatic synthesis of chiral hydroxy compounds using immobilized glucose dehydrogenase from bacillus cereus for nad(p)h regeneration

Immobilized thermostable glucose dehydrogenase from the mesophile Bacillus ce-reus has been prepared and used to catalyze the regeneration of NAD(P)H in enzymatic syntheses. The thermal stability of the immobilized enzyme in aqueous buffer containing NaCl is about 50,000 times that of the free enzyme. The half-life of the enzyme at 60°C is 30 minutes for the free form and more than 30 days for the immobilized form. We have used this cofactor regeneration system in the synthesis of a number of useful chiral hydroxy compounds.