TY - GEN
T1 - Estimating the expression of transcript isoforms from mRNA-seq via nonnegative least squares
AU - Kim, Hyunsoo
AU - Bi, Yingtao
AU - Davuluri, Ramana V.
PY - 2010
Y1 - 2010
N2 - mRNA-Seq is an emerging massive parallel sequencing based technology for identification and quantification of gene transcripts. Although gene level expression can easily be estimated from mRNA-Seq data, estimating the isoform level expression poses serious problems, and appropriate methods are required. In this paper, we introduce a mathematical method to estimate transcript isoform concentrations, i.e. transcript isoform expression estimation via nonnegative least squares (TIEE/NLS).
AB - mRNA-Seq is an emerging massive parallel sequencing based technology for identification and quantification of gene transcripts. Although gene level expression can easily be estimated from mRNA-Seq data, estimating the isoform level expression poses serious problems, and appropriate methods are required. In this paper, we introduce a mathematical method to estimate transcript isoform concentrations, i.e. transcript isoform expression estimation via nonnegative least squares (TIEE/NLS).
KW - Component
KW - mRNA-Seq
KW - Nonnegative least squares
KW - Transcript isoform
KW - Transcript isoform expression estimation
UR - https://www.scopus.com/pages/publications/77956161101
U2 - 10.1109/BIBE.2010.61
DO - 10.1109/BIBE.2010.61
M3 - Conference contribution
AN - SCOPUS:77956161101
SN - 9780769540832
T3 - 10th IEEE International Conference on Bioinformatics and Bioengineering 2010, BIBE 2010
SP - 296
EP - 297
BT - 10th IEEE International Conference on Bioinformatics and Bioengineering 2010, BIBE 2010
T2 - 10th IEEE International Conference on Bioinformatics and Bioengineering, BIBE-2010
Y2 - 31 May 2010 through 3 June 2010
ER -