Extraordinary Acoustic Raman Spectroscopy of PR65

Elham Babaei; Ghazal Hajisalem; Shohei Iwamoto; Burak Kaynak; Pemra Doruker; Mohsin M. Naqvi; Janet Kumita; Feng Yu Wang; Jhih Hong Cheng; Che Min Wu; Shang Hua Yang; Ivet Bahar; Laura Itzhaki; Reuven Gordon

doi:10.1109/NMDC50713.2021.9677469

Extraordinary Acoustic Raman Spectroscopy of PR65

Elham Babaei
, Ghazal Hajisalem
, Shohei Iwamoto
, Burak Kaynak
, Pemra Doruker
, Mohsin M. Naqvi
, Janet Kumita
, Feng Yu Wang
, Jhih Hong Cheng
, Che Min Wu
, Shang Hua Yang
, Ivet Bahar
, Laura Itzhaki
, Reuven Gordon

Laufer Center for Physical and Quantitative Biology

University of Victoria BC
University of Pittsburgh
University of Cambridge
National Tsing Hua University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Using double nanohole (DNH) optical tweezer setup and applying two lasers, we trapped and excited acoustic vibration modes of the PR65 subunit within the protein phosphatase 2A (PP2A). Our experiment shows that the vibrational modes are at frequencies of 24.26 GHz, 32.80 GHz, 48.47 GHz, 57.97 GHz which matches to the normal mode analysis results. This technique is single molecule and label free compared to typical methods used for characterization of biomolecules. Characterizing PR65 can be used as a paradigm for understanding the structural regulation of many repeat proteins.

Original language	English
Title of host publication	2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781665418928
DOIs	https://doi.org/10.1109/NMDC50713.2021.9677469
State	Published - 2021
Event	16th IEEE Nanotechnology Materials and Devices Conference, NMDC 2021 - Vancouver, Canada Duration: Dec 12 2021 → Dec 15 2021

Publication series

Name	2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021

Conference

Conference	16th IEEE Nanotechnology Materials and Devices Conference, NMDC 2021
Country/Territory	Canada
City	Vancouver
Period	12/12/21 → 12/15/21

Keywords

Acoustic Raman spectroscopy
DNH optical tweezer
PR65 protein
trapping

Access to Document

10.1109/NMDC50713.2021.9677469

Cite this

Babaei, E., Hajisalem, G., Iwamoto, S., Kaynak, B., Doruker, P., Naqvi, M. M., Kumita, J., Wang, F. Y., Cheng, J. H., Wu, C. M., Yang, S. H., Bahar, I., Itzhaki, L., & Gordon, R. (2021). Extraordinary Acoustic Raman Spectroscopy of PR65. In 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021 (2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NMDC50713.2021.9677469

@inproceedings{2c7394fd7ef84d21bc5f8533e8297625,

title = "Extraordinary Acoustic Raman Spectroscopy of PR65",

abstract = "Using double nanohole (DNH) optical tweezer setup and applying two lasers, we trapped and excited acoustic vibration modes of the PR65 subunit within the protein phosphatase 2A (PP2A). Our experiment shows that the vibrational modes are at frequencies of 24.26 GHz, 32.80 GHz, 48.47 GHz, 57.97 GHz which matches to the normal mode analysis results. This technique is single molecule and label free compared to typical methods used for characterization of biomolecules. Characterizing PR65 can be used as a paradigm for understanding the structural regulation of many repeat proteins.",

keywords = "Acoustic Raman spectroscopy, DNH optical tweezer, PR65 protein, trapping",

author = "Elham Babaei and Ghazal Hajisalem and Shohei Iwamoto and Burak Kaynak and Pemra Doruker and Naqvi, \{Mohsin M.\} and Janet Kumita and Wang, \{Feng Yu\} and Cheng, \{Jhih Hong\} and Wu, \{Che Min\} and Yang, \{Shang Hua\} and Ivet Bahar and Laura Itzhaki and Reuven Gordon",

note = "Publisher Copyright: {\textcopyright} 2021 IEEE.; 16th IEEE Nanotechnology Materials and Devices Conference, NMDC 2021 ; Conference date: 12-12-2021 Through 15-12-2021",

year = "2021",

doi = "10.1109/NMDC50713.2021.9677469",

language = "English",

series = "2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

booktitle = "2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021",

}

Babaei, E, Hajisalem, G, Iwamoto, S, Kaynak, B, Doruker, P, Naqvi, MM, Kumita, J, Wang, FY, Cheng, JH, Wu, CM, Yang, SH, Bahar, I, Itzhaki, L & Gordon, R 2021, Extraordinary Acoustic Raman Spectroscopy of PR65. in 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021. 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021, Institute of Electrical and Electronics Engineers Inc., 16th IEEE Nanotechnology Materials and Devices Conference, NMDC 2021, Vancouver, Canada, 12/12/21. https://doi.org/10.1109/NMDC50713.2021.9677469

Extraordinary Acoustic Raman Spectroscopy of PR65. / Babaei, Elham; Hajisalem, Ghazal; Iwamoto, Shohei et al.
2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021. Institute of Electrical and Electronics Engineers Inc., 2021. (2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Extraordinary Acoustic Raman Spectroscopy of PR65

AU - Babaei, Elham

AU - Hajisalem, Ghazal

AU - Iwamoto, Shohei

AU - Kaynak, Burak

AU - Doruker, Pemra

AU - Naqvi, Mohsin M.

AU - Kumita, Janet

AU - Wang, Feng Yu

AU - Cheng, Jhih Hong

AU - Wu, Che Min

AU - Yang, Shang Hua

AU - Bahar, Ivet

AU - Itzhaki, Laura

AU - Gordon, Reuven

PY - 2021

Y1 - 2021

N2 - Using double nanohole (DNH) optical tweezer setup and applying two lasers, we trapped and excited acoustic vibration modes of the PR65 subunit within the protein phosphatase 2A (PP2A). Our experiment shows that the vibrational modes are at frequencies of 24.26 GHz, 32.80 GHz, 48.47 GHz, 57.97 GHz which matches to the normal mode analysis results. This technique is single molecule and label free compared to typical methods used for characterization of biomolecules. Characterizing PR65 can be used as a paradigm for understanding the structural regulation of many repeat proteins.

AB - Using double nanohole (DNH) optical tweezer setup and applying two lasers, we trapped and excited acoustic vibration modes of the PR65 subunit within the protein phosphatase 2A (PP2A). Our experiment shows that the vibrational modes are at frequencies of 24.26 GHz, 32.80 GHz, 48.47 GHz, 57.97 GHz which matches to the normal mode analysis results. This technique is single molecule and label free compared to typical methods used for characterization of biomolecules. Characterizing PR65 can be used as a paradigm for understanding the structural regulation of many repeat proteins.

KW - Acoustic Raman spectroscopy

KW - DNH optical tweezer

KW - PR65 protein

KW - trapping

UR - https://www.scopus.com/pages/publications/85125298925

U2 - 10.1109/NMDC50713.2021.9677469

DO - 10.1109/NMDC50713.2021.9677469

M3 - Conference contribution

AN - SCOPUS:85125298925

T3 - 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021

BT - 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 16th IEEE Nanotechnology Materials and Devices Conference, NMDC 2021

Y2 - 12 December 2021 through 15 December 2021

ER -

Babaei E, Hajisalem G, Iwamoto S, Kaynak B, Doruker P, Naqvi MM et al. Extraordinary Acoustic Raman Spectroscopy of PR65. In 2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021. Institute of Electrical and Electronics Engineers Inc. 2021. (2021 IEEE 16th Nanotechnology Materials and Devices Conference, NMDC 2021). doi: 10.1109/NMDC50713.2021.9677469

Extraordinary Acoustic Raman Spectroscopy of PR65

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Keywords

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