@inproceedings{56dc2e7b32c64ff281ea15d5441ab9e8,
title = "Flow-through electroporation for transfection based on low-frequency AC voltage",
abstract = "Electroporation has been demonstrated to be an efficient technique for gene transfection on mammalian cells. However, traditional technique employing direct current (DC) electrical pulses has the problem of too much electrolysis and low cell viability, which limits the application in gene delivery study. We demonstrate here flow-through electroporation for transfection based on low-frequency alternating current (AC) source. We investigate the effects of the AC frequency on the cell size expansion, permeability, transfection efficiency and cell viability. Our technique provides a potential novel way for a convenient high throughput gene delivery.",
keywords = "AC voltage, Electroporation, Low-frequency, Transfection",
author = "Y. Zhan and J. Wang and N. Bao and T. Geng and C. Lu",
year = "2010",
language = "English",
isbn = "9781618390622",
series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
pages = "1550--1552",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
note = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",
}