Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

K. E. Hechemy; H. L. Harris; J. A. Wethers; R. W. Stevens; B. R. Stock; A. A. Reilly; J. L. Benach

doi:10.1128/jcm.27.8.1854-1858.1989

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

K. E. Hechemy
, H. L. Harris
, J. A. Wethers
, R. W. Stevens
, B. R. Stock
, A. A. Reilly
, J. L. Benach

Office of the Dean of Medicine

Wadsworth Center for Laboratories and Research

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

Original language	English
Pages (from-to)	1854-1858
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	27
Issue number	8
DOIs	https://doi.org/10.1128/jcm.27.8.1854-1858.1989
State	Published - 1989

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10.1128/jcm.27.8.1854-1858.1989

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title = "Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi",

abstract = "Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.",

author = "Hechemy, \{K. E.\} and Harris, \{H. L.\} and Wethers, \{J. A.\} and Stevens, \{R. W.\} and Stock, \{B. R.\} and Reilly, \{A. A.\} and Benach, \{J. L.\}",

year = "1989",

doi = "10.1128/jcm.27.8.1854-1858.1989",

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T1 - Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

AU - Hechemy, K. E.

AU - Harris, H. L.

AU - Wethers, J. A.

AU - Stevens, R. W.

AU - Stock, B. R.

AU - Reilly, A. A.

AU - Benach, J. L.

PY - 1989

Y1 - 1989

N2 - Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

AB - Sodium deoxycholate-solubilized Borrelia burgdorferi antigen was prepared for use in a solid-phase fluoroimmunoassay (FIA-L) to detect antibodies in Lyme disease. Serum specimens were tested by FIA-L and by a microimmunofluorescence test. The FIA-L results are comparable to those of the standard microimmunofluorescence test. The overall agreement was 0.98. Moreover, the FIA-L procedure is simple and rapid; fluorescence is objectively determined and is proportional to antibody titer.

UR - https://www.scopus.com/pages/publications/0024403668

U2 - 10.1128/jcm.27.8.1854-1858.1989

DO - 10.1128/jcm.27.8.1854-1858.1989

M3 - Article

C2 - 2671034

AN - SCOPUS:0024403668

SN - 0095-1137

VL - 27

SP - 1854

EP - 1858

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 8

ER -

Fluoroimmunoassay studies with solubilized antigens from Borrelia burgdorferi

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