Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer

Pamela L. Paris; Yasuko Kobayashi; Qiang Zhao; Wei Zeng; Shivaranjani Sridharan; Tina Fan; Howard L. Adler; Emmanuel R. Yera; M. H. Zarrabi; Stanley Zucker; Jeffry Simko; Wen Tien Chen; Jonathan Rosenberg

doi:10.1016/j.canlet.2008.12.007

Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer

Pamela L. Paris
, Yasuko Kobayashi
, Qiang Zhao
, Wei Zeng
, Shivaranjani Sridharan
, Tina Fan
, Howard L. Adler
, Emmanuel R. Yera
, M. H. Zarrabi
, Stanley Zucker
, Jeffry Simko
, Wen Tien Chen
, Jonathan Rosenberg

Urology

University of California at San Francisco
Stony Brook University
Vitatex Inc.
VA Medical Center

Research output: Contribution to journal › Article › peer-review

85 Scopus citations

Abstract

Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.

Original language	English
Pages (from-to)	164-173
Number of pages	10
Journal	Cancer Letters
Volume	277
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2008.12.007
State	Published - May 18 2009

Keywords

Blood micrometastases
Circulating tumor cells
Metastasis
Prostate cancer

Access to Document

10.1016/j.canlet.2008.12.007

Cite this

Paris, P. L., Kobayashi, Y., Zhao, Q., Zeng, W., Sridharan, S., Fan, T., Adler, H. L., Yera, E. R., Zarrabi, M. H., Zucker, S., Simko, J., Chen, W. T., & Rosenberg, J. (2009). Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer. Cancer Letters, 277(2), 164-173. https://doi.org/10.1016/j.canlet.2008.12.007

@article{dbd8aa1d74df4f639697752339beb96b,

title = "Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer",

abstract = "Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.",

keywords = "Blood micrometastases, Circulating tumor cells, Metastasis, Prostate cancer",

author = "Paris, \{Pamela L.\} and Yasuko Kobayashi and Qiang Zhao and Wei Zeng and Shivaranjani Sridharan and Tina Fan and Adler, \{Howard L.\} and Yera, \{Emmanuel R.\} and Zarrabi, \{M. H.\} and Stanley Zucker and Jeffry Simko and Chen, \{Wen Tien\} and Jonathan Rosenberg",

year = "2009",

month = may,

day = "18",

doi = "10.1016/j.canlet.2008.12.007",

language = "English",

volume = "277",

pages = "164--173",

journal = "Cancer Letters",

issn = "0304-3835",

number = "2",

}

TY - JOUR

T1 - Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer

AU - Paris, Pamela L.

AU - Kobayashi, Yasuko

AU - Zhao, Qiang

AU - Zeng, Wei

AU - Sridharan, Shivaranjani

AU - Fan, Tina

AU - Adler, Howard L.

AU - Yera, Emmanuel R.

AU - Zarrabi, M. H.

AU - Zucker, Stanley

AU - Simko, Jeffry

AU - Chen, Wen Tien

AU - Rosenberg, Jonathan

PY - 2009/5/18

Y1 - 2009/5/18

N2 - Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.

AB - Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.

KW - Blood micrometastases

KW - Circulating tumor cells

KW - Metastasis

KW - Prostate cancer

UR - https://www.scopus.com/pages/publications/62749092492

U2 - 10.1016/j.canlet.2008.12.007

DO - 10.1016/j.canlet.2008.12.007

M3 - Article

C2 - 19162393

AN - SCOPUS:62749092492

SN - 0304-3835

VL - 277

SP - 164

EP - 173

JO - Cancer Letters

JF - Cancer Letters

IS - 2

ER -

Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this