Glucocorticoids modulate mRNA levels for G-protein β-subunits

M. Ros; D. C. Watkins; P. J. Rapiejko; C. C. Malbon

doi:10.1042/bj2600271

Glucocorticoids modulate mRNA levels for G-protein β-subunits

M. Ros
, D. C. Watkins
, P. J. Rapiejko
, C. C. Malbon

Pharmacological Sciences

Stony Brook University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein β-subunits (G(β)) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G(β) [ 5.8 ± 0.4 amol/μg of RNA (n = 5) in control fat-cells]. G(β) mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G(β) mRNA levels by 50%.

Original language	English
Pages (from-to)	271-275
Number of pages	5
Journal	Biochemical Journal
Volume	260
Issue number	1
DOIs	https://doi.org/10.1042/bj2600271
State	Published - 1989

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abstract = "Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein β-subunits (G(β)) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G(β) [ 5.8 ± 0.4 amol/μg of RNA (n = 5) in control fat-cells]. G(β) mRNA levels decrease by 20\% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G(β) mRNA levels by 50\%.",

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N2 - Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein β-subunits (G(β)) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G(β) [ 5.8 ± 0.4 amol/μg of RNA (n = 5) in control fat-cells]. G(β) mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G(β) mRNA levels by 50%.

AB - Adrenalectomy decreases, whereas glucocorticoid treatment increases, the steady-state levels of G-protein β-subunits (G(β)) in rat fat-cells. A DNA-excess solution-hybridization assay was established to define the steady-state mRNA levels for G(β) [ 5.8 ± 0.4 amol/μg of RNA (n = 5) in control fat-cells]. G(β) mRNA levels decrease by 20% after adrenalectomy; dexamethasone treatment reverses the decline. Dexamethasone treatment itself increases G(β) mRNA levels by 50%.

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JO - Biochemical Journal

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Glucocorticoids modulate mRNA levels for G-protein β-subunits

Abstract

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