High-content analysis of cancer genome DNA alterations

Yan Y. Degenhardt; Richard Wooster; Richard W. McCombie; Robert Lucito; Scott Powers

doi:10.1016/j.gde.2008.01.005

High-content analysis of cancer genome DNA alterations

Yan Y. Degenhardt
, Richard Wooster
, Richard W. McCombie
, Robert Lucito
, Scott Powers

Pathology

GlaxoSmithKline
Cold Spring Harbor Laboratory

Research output: Contribution to journal › Review article › peer-review

11 Scopus citations

Abstract

New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.

Original language	English
Pages (from-to)	68-72
Number of pages	5
Journal	Current Opinion in Genetics and Development
Volume	18
Issue number	1
DOIs	https://doi.org/10.1016/j.gde.2008.01.005
State	Published - Feb 2008

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10.1016/j.gde.2008.01.005

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abstract = "New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.",

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AU - Wooster, Richard

AU - McCombie, Richard W.

AU - Lucito, Robert

AU - Powers, Scott

PY - 2008/2

Y1 - 2008/2

N2 - New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.

AB - New technologies as well as concerted brute-force approaches have increased the content (number of genes) that can be characterized for genomic DNA alterations. Recent advances include the detection of activating point mutations in key kinase genes (BRAF, EGFR, and PIK3CA) in multiple cancer types: preliminary insight into the entire repertoire of genes that can be mutated in cancer; the discovery of new oncogenes by high-resolution profiling of DNA copy number alterations; and the bioinformatic-driven discovery of oncogenic gene fusions. High-content promoter methylation detection systems have been used to discover additional methylated genes and have provided evidence for a stem cell origin for certain tumors. Some of these advances have had significant impact on the development and clinical testing of new therapeutics.

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DO - 10.1016/j.gde.2008.01.005

M3 - Review article

C2 - 18339543

AN - SCOPUS:43749092206

SN - 0959-437X

VL - 18

SP - 68

EP - 72

JO - Current Opinion in Genetics and Development

JF - Current Opinion in Genetics and Development

IS - 1

ER -

High-content analysis of cancer genome DNA alterations

Abstract

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