TY - GEN
T1 - Hyaluronic acid -gelatin fibrous scaffold produced by electrospinning of their aqueous solution for tissue engineering applications
AU - Liu, Ying
AU - Clark, Richard A.F.
AU - Huang, Lei
AU - Rafailovich, Miriam H.
PY - 2009
Y1 - 2009
N2 - A three-dimensional (3D) hyaluronic acid (HA) and gelatin (Gtn) fibrous scaffold was successfully fabricated to mimic the architecture of natural extracellular matrix (ECM) based on electrospinning. Thiolated HA and Gtn derivatives, HA-DTPH and Gtn-DTPH, were synthesized and electrospun to form composite 3D fibrous scaffolds. In order to facilitate the fiber formation during electrospinning, Poly (ethylene oxide) (PEO) was added into the aqueous solution of HA-DTPH and Gtn-DTPH at an optimal weight ratio of 1:1.2. The electrospun HA-Gtn/PEO blend scaffold was subsequently cross-linked through Poly (ethylene glycol)-diacrylate (PEGDA) mediated conjugate addition. PEO was then extracted in deionized (DI) water to obtain an electrospun HA-Gtn fibrous scaffold. Adult human dermal fibroblasts (AHDFs) were seeded on HA-Gtn fibrous scaffold for 24h in vitro. Laser scanning confocal microscopy (LSCM) revealed that the AHDFs attached to the scaffold and spread, which suggests potential applications of HA-Gtn firbous scaffolds in tissue regeneration.
AB - A three-dimensional (3D) hyaluronic acid (HA) and gelatin (Gtn) fibrous scaffold was successfully fabricated to mimic the architecture of natural extracellular matrix (ECM) based on electrospinning. Thiolated HA and Gtn derivatives, HA-DTPH and Gtn-DTPH, were synthesized and electrospun to form composite 3D fibrous scaffolds. In order to facilitate the fiber formation during electrospinning, Poly (ethylene oxide) (PEO) was added into the aqueous solution of HA-DTPH and Gtn-DTPH at an optimal weight ratio of 1:1.2. The electrospun HA-Gtn/PEO blend scaffold was subsequently cross-linked through Poly (ethylene glycol)-diacrylate (PEGDA) mediated conjugate addition. PEO was then extracted in deionized (DI) water to obtain an electrospun HA-Gtn fibrous scaffold. Adult human dermal fibroblasts (AHDFs) were seeded on HA-Gtn fibrous scaffold for 24h in vitro. Laser scanning confocal microscopy (LSCM) revealed that the AHDFs attached to the scaffold and spread, which suggests potential applications of HA-Gtn firbous scaffolds in tissue regeneration.
UR - https://www.scopus.com/pages/publications/72849146935
M3 - Conference contribution
AN - SCOPUS:72849146935
SN - 9781605111124
T3 - Materials Research Society Symposium Proceedings
SP - 131
EP - 136
BT - Advances in Material Design for Regenerative Medicine, Drug Delivery and Targeting/Imaging
T2 - MRS Fall Meeting 2008: Advances in Material Design for Regenerative Medicine, Drug Delivery and Targeting/Imaging
Y2 - 1 December 2008 through 3 December 2008
ER -