@inbook{8f57428913204959bea4197d3fd11663,
title = "Identifying Inhibitor Targets in Mycobacteria by Activity-Based Probe Profiling",
abstract = "Activity-based protein profiling (ABPP) enables the detection of protein reactivity across entire proteomes. Here, we describe the application of ABPP to the identification of serine hydrolase inhibitor targets in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis in humans. We highlight the adaptation of stable isotope labeling of amino acids (SILAC) to Mtb using a modified growth medium with an isotopically labeled sole nitrogen source and detail the resulting special requirements for proteomics analysis.",
keywords = "Activity-based protein profiling, Inhibitor, Mycobacteria, Proteomics, SILAC, Tuberculosis",
author = "Neetika Jaisinghani and Isabel Sakarin and Patel, \{Hiren V.\} and Michael Li and Previti, \{Mary L.\} and Seeliger, \{Jessica C.\}",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",
year = "2025",
doi = "10.1007/978-1-0716-4502-4\_7",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "139--154",
booktitle = "Methods in Molecular Biology",
}