In-situ photochemical fluorimetry of tyrosine and its determination

Weiping Cai; Xisheng Wang; Qingzhi Zhu; Zhihong Wang; Jingou Xu

In-situ photochemical fluorimetry of tyrosine and its determination

Weiping Cai
, Xisheng Wang
, Qingzhi Zhu
, Zhihong Wang
, Jingou Xu

Office of the Dean SOMAS

Xiamen University

Research output: Contribution to journal › Article › peer-review

Abstract

Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH₃-NH₄Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 × 10^-7 ∼ 2.0 × 10^-5 mol/L tyrosine. The detection limit is 3.2 × 10^-9 mol/L and relative standard deviation is 1.7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.

Original language	English
Pages (from-to)	1537
Number of pages	1
Journal	Fenxi Huaxue
Volume	28
Issue number	12
State	Published - 2000

Keywords

Fluorimetry
Photochemical reaction
Tyrosine

Cite this

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title = "In-situ photochemical fluorimetry of tyrosine and its determination",

abstract = "Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 × 10-7 ∼ 2.0 × 10-5 mol/L tyrosine. The detection limit is 3.2 × 10-9 mol/L and relative standard deviation is 1.7\%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.",

keywords = "Fluorimetry, Photochemical reaction, Tyrosine",

author = "Weiping Cai and Xisheng Wang and Qingzhi Zhu and Zhihong Wang and Jingou Xu",

year = "2000",

language = "English",

volume = "28",

pages = "1537",

journal = "Fenxi Huaxue",

issn = "0253-3820",

number = "12",

}

TY - JOUR

T1 - In-situ photochemical fluorimetry of tyrosine and its determination

AU - Cai, Weiping

AU - Wang, Xisheng

AU - Zhu, Qingzhi

AU - Wang, Zhihong

AU - Xu, Jingou

PY - 2000

Y1 - 2000

N2 - Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 × 10-7 ∼ 2.0 × 10-5 mol/L tyrosine. The detection limit is 3.2 × 10-9 mol/L and relative standard deviation is 1.7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.

AB - Tyrosine can produce fluorescent dimers under irradiation with 275nm UV-light in NH3-NH4Cl buffer. Besed on this fact, a new photochemical fluorimetry in-situ was developed for determination of tyrosine. The linear relationship is over the range of 2.0 × 10-7 ∼ 2.0 × 10-5 mol/L tyrosine. The detection limit is 3.2 × 10-9 mol/L and relative standard deviation is 1.7%. This method is simple and rapid, and has better selectivity for other amino-acids. It has been applied to the determination of tyrosine in beer and amino-acid injections. Mechanism of the photochemical reaction was discussed.

KW - Fluorimetry

KW - Photochemical reaction

KW - Tyrosine

UR - https://www.scopus.com/pages/publications/9644301285

M3 - Article

AN - SCOPUS:9644301285

SN - 0253-3820

VL - 28

SP - 1537

JO - Fenxi Huaxue

JF - Fenxi Huaxue

IS - 12

ER -

In-situ photochemical fluorimetry of tyrosine and its determination

Abstract

Keywords

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