In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

Qing Zhi Zhu; Jin Gou Xu; Xiang Qun Guo; Xue Ying Zheng; Wen You Li; Yi Bing Zhao

doi:10.1016/S1386-1425(97)00075-9

In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

Qing Zhi Zhu
, Jin Gou Xu
, Xiang Qun Guo
, Xue Ying Zheng
, Wen You Li
, Yi Bing Zhao

Office of the Dean SOMAS

Xiamen University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorimetric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ were investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10^-7 M BSA. The detection limit was 1.2 × 10^-10 M BSA and the relative standard deviation was 2.24% for 1.42 × 10^-8 M BSA (n = 7).

Original language	English
Pages (from-to)	1883-1887
Number of pages	5
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	53
Issue number	11
DOIs	https://doi.org/10.1016/S1386-1425(97)00075-9
State	Published - Oct 1 1997

Keywords

9,10-Anthraquinone
Bovine serum albumin detection
Fluorimetry
Photochemical reaction

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10.1016/S1386-1425(97)00075-9

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title = "In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin",

abstract = "Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorimetric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ were investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24\% for 1.42 × 10-8 M BSA (n = 7).",

keywords = "9,10-Anthraquinone, Bovine serum albumin detection, Fluorimetry, Photochemical reaction",

author = "Zhu, \{Qing Zhi\} and Xu, \{Jin Gou\} and Guo, \{Xiang Qun\} and Zheng, \{Xue Ying\} and Li, \{Wen You\} and Zhao, \{Yi Bing\}",

year = "1997",

month = oct,

day = "1",

doi = "10.1016/S1386-1425(97)00075-9",

language = "English",

volume = "53",

pages = "1883--1887",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

issn = "1386-1425",

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TY - JOUR

T1 - In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

AU - Zhu, Qing Zhi

AU - Xu, Jin Gou

AU - Guo, Xiang Qun

AU - Zheng, Xue Ying

AU - Li, Wen You

AU - Zhao, Yi Bing

PY - 1997/10/1

Y1 - 1997/10/1

N2 - Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorimetric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ were investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24% for 1.42 × 10-8 M BSA (n = 7).

AB - Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorimetric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ were investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24% for 1.42 × 10-8 M BSA (n = 7).

KW - 9,10-Anthraquinone

KW - Bovine serum albumin detection

KW - Fluorimetry

KW - Photochemical reaction

UR - https://www.scopus.com/pages/publications/0031572393

U2 - 10.1016/S1386-1425(97)00075-9

DO - 10.1016/S1386-1425(97)00075-9

M3 - Article

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AN - SCOPUS:0031572393

SN - 1386-1425

VL - 53

SP - 1883

EP - 1887

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

IS - 11

ER -

In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

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Keywords

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