In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

Quing Zhi Zhu; Jin Gou Xu; Xuang Qun Guo; Xue Ying Zheng; Wen You Li; Yi Bing Zhao

doi:10.1016/S1386-1425(97)00123-6

In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

Quing Zhi Zhu
, Jin Gou Xu
, Xuang Qun Guo
, Xue Ying Zheng
, Wen You Li
, Yi Bing Zhao

Office of the Dean SOMAS

Xiamen University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorometric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ was investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10^-7 M BSA. The detection limit was 1.2 × 10^-10 M BSA and the relative standard deviation was 2.24% for the 1.42 × 10^-8 M BSA (n = 7).

Original language	English
Pages (from-to)	2195-2200
Number of pages	6
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	53
Issue number	12
DOIs	https://doi.org/10.1016/S1386-1425(97)00123-6
State	Published - Oct 15 1997

Keywords

9,10-Anthraquinone
BSA detection
Fluorimetry
Photochemical reaction

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10.1016/S1386-1425(97)00123-6

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title = "In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin",

abstract = "Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorometric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ was investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24\% for the 1.42 × 10-8 M BSA (n = 7).",

keywords = "9,10-Anthraquinone, BSA detection, Fluorimetry, Photochemical reaction",

author = "Zhu, \{Quing Zhi\} and Xu, \{Jin Gou\} and Guo, \{Xuang Qun\} and Zheng, \{Xue Ying\} and Li, \{Wen You\} and Zhao, \{Yi Bing\}",

year = "1997",

month = oct,

day = "15",

doi = "10.1016/S1386-1425(97)00123-6",

language = "English",

volume = "53",

pages = "2195--2200",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

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TY - JOUR

T1 - In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

AU - Zhu, Quing Zhi

AU - Xu, Jin Gou

AU - Guo, Xuang Qun

AU - Zheng, Xue Ying

AU - Li, Wen You

AU - Zhao, Yi Bing

PY - 1997/10/15

Y1 - 1997/10/15

N2 - Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorometric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ was investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24% for the 1.42 × 10-8 M BSA (n = 7).

AB - Bovine serum albumin (BSA) labeled with 9,10-anthraquinone (AQ) shows a greatly enhanced photochemical fluorometric activity compared with that of free AQ. The spectral characteristics of the photoreduction product of conjugated AQ was investigated and large blue shifts in the excitation and emission bands compared with those of free AQ were observed. The enhancement in the photochemical reactivity can be employed for sensitive detection of labeled BSA by a simple in-situ photochemical kinetic fluorimetric method. The kinetic behavior of the photochemical reaction and the effects of some experimental conditions were investigated. The calibration graph was linear over the range 0-1.8 × 10-7 M BSA. The detection limit was 1.2 × 10-10 M BSA and the relative standard deviation was 2.24% for the 1.42 × 10-8 M BSA (n = 7).

KW - 9,10-Anthraquinone

KW - BSA detection

KW - Fluorimetry

KW - Photochemical reaction

UR - https://www.scopus.com/pages/publications/0031254695

U2 - 10.1016/S1386-1425(97)00123-6

DO - 10.1016/S1386-1425(97)00123-6

M3 - Article

AN - SCOPUS:0031254695

SN - 1386-1425

VL - 53

SP - 2195

EP - 2200

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

IS - 12

ER -

In-situ photochemical spectrofluorimetric detection of 9,10-anthraquinone-labeled bovine serum albumin

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Keywords

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