In vitro selection of a single-stranded dna molecular recognition element specific for bromacil

Ryan M. Williams; Amanda R. Kulick; Srilakshmi Yedlapalli; Louisa Battistella; Cyrus J. Hajiran; Letha J. Sooter

doi:10.1155/2014/102968

In vitro selection of a single-stranded dna molecular recognition element specific for bromacil

Ryan M. Williams
, Amanda R. Kulick
, Srilakshmi Yedlapalli
, Louisa Battistella
, Cyrus J. Hajiran
, Letha J. Sooter

Nephrology and Hypertension

West Virginia University

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.

Original language	English
Article number	102968
Journal	Journal of Nucleic Acids
Volume	2014
DOIs	https://doi.org/10.1155/2014/102968
State	Published - 2014

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abstract = "Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.",

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AU - Kulick, Amanda R.

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AU - Battistella, Louisa

AU - Hajiran, Cyrus J.

AU - Sooter, Letha J.

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AB - Bromacil is a widely used herbicide that is known to contaminate environmental systems. Due to the hazards it presents and inefficient detection methods, it is necessary to create a rapid and efficient sensing device. Towards this end, we have utilized a stringent in vitro selection method to identify single-stranded DNA molecular recognition elements (MRE) specific for bromacil. We have identified one MRE with high affinity (K d = 9.6 nM) and specificity for bromacil compared to negative targets of selection and other pesticides. The selected ssDNA MRE will be useful as the sensing element in a field-deployable bromacil detection device.

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VL - 2014

JO - Journal of Nucleic Acids

JF - Journal of Nucleic Acids

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ER -

In vitro selection of a single-stranded dna molecular recognition element specific for bromacil

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