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In vitro studies of membrane permeability induced by amyloidogenic polypeptides using large unilamellar vesicles

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

The process of amyloid formation is cytotoxic and contributes to a wide range of human diseases, but the mechanisms of amyloid-induced cytotoxicity are not well understood. It has been proposed that amyloidogenic peptides exert their toxiceffects by damaging membranes. Membrane disruption is clearly not the only mechanism of toxicity, but the literature suggests that loss of membrane integrity may be a contributing factor. In this chapter we describe the measurement of in vitro membrane leakage induced by amyloidogenic proteinsvia the use of model vesicles.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages283-290
Number of pages8
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1345
ISSN (Print)1064-3745

Keywords

  • Amyloid
  • Cytotoxicity
  • Islet amyloid polypeptide
  • Membrane disruption
  • Membrane leakage

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